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JoVE Journal
Bioengineering

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Rotating Cell Culture Systems for Human Cell Culture
 

Rotating Cell Culture Systems for Human Cell Culture: Human Trophoblast Cells as a Model

Article doi: 10.3791/3367
January 18th, 2012

Summary January 18th, 2012

Traditional, two dimensional cell culture techniques often result in altered characteristics with respect to differentiation markers, cytokines and growth factors. Three-dimensional cell culture in the rotating cell culture system (RCCS) reestablishes expression of many of these factors as shown here with an extravillous trophoblast cell line.

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