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Bioengineering

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JoVE Journal Bioengineering
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Rotating Cell Culture Systems for Human Cell Culture: Human Trophoblast Cells as a Model

Article DOI: 10.3791/3367
January 18th, 2012 •
*1, *1,2, 3, 1, 1
1Department of Microbiology and Immunology, Tulane University Medical School, 2Physician/Scientist Program, Tulane University Medical School, 3Department of Molecular and Cellular Biology, Baylor College of Medicine
* These authors contributed equally

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January 18th, 2012

Traditional, two dimensional cell culture techniques often result in altered characteristics with respect to differentiation markers, cytokines and growth factors. Three-dimensional cell culture in the rotating cell culture system (RCCS) reestablishes expression of many of these factors as shown here with an extravillous trophoblast cell line.

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