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Rotating Cell Culture Systems for Human Cell Culture: Human Trophoblast Cells as a Model
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Chapters
- 00:05Title
- 01:04EVT Cells and Collagen Bead Incubation
- 02:13Loading the Rotating Cell Culture System (RCCS)
- 03:41Changing the Media
- 04:37Collecting Propagated Cells
- 05:39SGHPL-4 Trophoblast Cells in a 3D Milieu
- 06:30Conclusion
Traditional, two dimensional cell culture techniques often result in altered characteristics with respect to differentiation markers, cytokines and growth factors. Three-dimensional cell culture in the rotating cell culture system (RCCS) reestablishes expression of many of these factors as shown here with an extravillous trophoblast cell line.
Tags
Rotating Cell Culture SystemsHuman Cell CultureHuman Trophoblast CellsModelPrimary CulturesExplant CulturesTrophoblast Cell LinesInvasion Of Uterine WallRemodeling Of Uterine Spiral ArteriesExtravillous Trophoblast CellsSuccessful Establishment Of PregnancyIn Vitro Cell Culture ModelsRotating Cell Culture System (RCCS)Morphological PropertiesPhenotypic PropertiesFunctional PropertiesEVT-like Cell LinesDifferentiation Of EVTsGene ExpressionMatrix Metalloproteinases (MMPs)Trophoblast DifferentiationSaint Georges Hospital Placental Cell Line-4 (SGHPL-4)RCCS Culture VesselThree-dimensional Environment
