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Human ES cells: Starting Culture from Frozen Cells
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Human ES cells: Starting Culture from Frozen Cells

Human ES cells: Starting Culture from Frozen Cells

DOI:
10.3791/86-v

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02:47 min

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November 09, 2006

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, ,
1Department of Molecular and Cell Biology,Harvard

Chapters

  • 00:03Prepare media
  • 00:17Take cells from freezer and thaw
  • 01:58Start culture

Summary

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Here we demonstrate how our lab begins a HuES human embryonic stem cell line culture from a frozen stock.

Tags

Human ES CellsCultureFrozen CellsEmbryonic Stem Cell LineHuES MediaMouse Embryonic Fibroblast Feeder CellsSerumCell DebrisLiquid Nitrogen Storage-80C FreezerThawingFreezing MediaDMSODifferentiationSuspensionSeedingMEF PlateIncubatorMedia Replacement

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