Cell Mapping
Cell Mapping - Related Videos
07:53
Single Cell Fate Mapping in Zebrafish
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Cited by 6 •
2011
A method is described to photoactivate single cells containing a caged fluorescent protein using two-photon absorption from a Ti:Sapphire femtosecond laser oscillator. To fate map the photoactivated cell, immunohistochemistry is used. This technique can be applied to any cell type.
06:58
Fate Mapping
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2023
Fate mapping is a technique used to understand how embryonic cells divide, differentiate, and migrate during development. In classic fate mapping experiments, cells in different areas of an embryo are labeled with a chemical dye and then tracked to determine which tissues or structures they form. Technological improvements now allow for individual cells to be marked and traced throughout embryonic development and adulthood. This video reviews the concepts behind fate mapping, and then details a...
15:08
Probing and Mapping Electrode Surfaces in Solid Oxide Fuel Cells
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Cited by 4 •
2012
We present a unique platform for characterizing electrode surfaces in solid oxide fuel cells (SOFCs) that allows simultaneous performance of multiple characterization techniques (e.g. in situ Raman spectroscopy and scanning probe microscopy alongside electrochemical measurements). Complementary information from these analyses may help to advance toward a more profound understanding of electrode reaction and degradation mechanisms, providing insights into rational design of better materials for...
11:03
Motor Maps
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2023
Source: Laboratories of Jonas T. Kaplan and Sarah I. Gimbel—University of Southern California One principle of brain organization is the topographic mapping of information. Especially in sensory and motor cortices, adjacent regions of the brain tend to represent information from adjacent parts of the body, resulting in maps of the body expressed on the surface of the brain. The primary sensory and motor maps in the brain surround a prominent sulcus known as the central sulcus. The cortex...
08:56
Fate Mapping of Human Embryonic Stem Cells by Teratoma Formation
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Cited by 26 •
2010
Directed differentiation of hESCs into specific cells has generated much interest in regenerative medicine. We provide a concise, step-by-step protocol for determining the in vivo fate of selected hESCs that provides a valuable tool for characterizing tissue-specific reagents for cell-based therapy.
FAQs
Cell mapping links what a cell is doing molecularly with where it is located and which neighboring cells surround it. Microscopy and molecular markers help distinguish cellular identities, while spatial transcriptomics associates gene-expression profiles with precise tissue positions. This combined view shows how physical arrangement supports tissue function and reveals relationships that molecular or visual data alone may miss.
Each approach contributes a different type of evidence. Microscopy shows cellular structure and location, molecular markers help identify cell types, and lineage tracing follows cellular origins or developmental paths. Spatial transcriptomics connects molecular profiles to tissue coordinates. Used together, these methods provide complementary information about identity, ancestry, position, and changing cellular states.
A cell’s position relative to other cells can help explain communication, tissue organization, and functional behavior. Mapping these relationships also supports analysis of how cells change during development or respond to injury and disease. Consequently, a map is not only a catalog of cell types; it can also provide a framework for studying interactions and biological changes across a tissue.
A study generally begins by examining a tissue, organism, or biological system with microscopy and selecting molecular markers that distinguish relevant cell types. Researchers may add lineage tracing to follow cellular origins and spatial transcriptomics to associate molecular profiles with locations. The resulting information is integrated to characterize cellular organization, relationships, and changes within the biological context being studied.
Cell mapping supports comparisons between healthy and diseased states by showing which cellular identities, positions, relationships, or molecular profiles differ. These comparisons can be applied to cancer biology and to responses following injury. Cellular atlases provide reference frameworks that help organize such observations, making it easier to relate disease-associated changes to normal tissue architecture.
In developmental biology, mapping can connect cellular locations and molecular profiles with developmental pathways, helping researchers examine how organized tissues emerge and change. In neuroscience, the same principles help characterize cellular organization and relationships within nervous-system tissues. Across both fields, combining spatial and molecular information provides context for interpreting cellular diversity and function.