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Chapters
- 00:05Title
- 01:25Two-photon Activation of Caged Fluorescein-dextran
- 05:03Immunodetection of Uncaged Fluorescein-dextran
- 06:04Photoactivation and Immunodetection of Caged Fluorescein-dextran
- 07:11Conclusion
A method is described to photoactivate single cells containing a caged fluorescent protein using two-photon absorption from a Ti:Sapphire femtosecond laser oscillator. To fate map the photoactivated cell, immunohistochemistry is used. This technique can be applied to any cell type.
Tags
Single Cell Fate MappingZebrafishDevelopmental BiologyFate MappingLineage TracingUndifferentiated Precursor CellsPhotoactivatable ProteinsFluorescence SpectrumUV ExcitationCell Tracing ProbesLong-term Cell TrackingCaged Fluorescein-dextranEmbryo Model SystemsUncaging Fluorescein-dextranSpatial ResolutionPhotoactivationNear-infrared Laser PulsesTitanium Sapphire Femtosecond LaserTwo-photon Confocal Microscopes
