Cell Replacement
Cell Replacement - Related Videos
06:47
Cryopreserving and Recovering of Human iPS Cells using Complete KnockOut Serum Replacement Feeder-Free Medium
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Cited by 12 •
2010
This protocol describes the detailed procedure for cryopreserving human iPS cells in KnockOut SR cryopreservation medium and recovering these cells in complete KnockOut SR Feeder Free (KSR-FF) medium or feeder-based KnockOut SR medium.
08:29
Feeder-Free Adaptation, Culture and Passaging of Human IPS Cells using Complete KnockOut Serum Replacement Feeder-Free Medium
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Cited by 14 •
2010
The following protocol provides instruction for adapting human induced Pluripotent Stem (iPS) Cells to feeder-free culture using complete KnockOut Serum Replacement Feeder-Free medium (KSR-FF). Once adapted, instructions for continual maintenance are also provided.
01:30
Continuous Renal Replacement Therapy
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2025
Continuous Renal Replacement Therapy, also known as CRRT, is a procedural treatment for acute kidney injury (AKI) that gradually removes uremic toxins and fluids while maintaining acid-base balance and stabilizing electrolytes. It is particularly useful for hemodynamically unstable patients. Unlike intermittent hemodialysis, which is faster, CRRT provides a gentler approach over 24 hours, closely mimicking the function of natural kidneys. However, CRRT is not ideal for patients with...
09:34
Preparation of Plasma Membrane Vesicles from Bone Marrow Mesenchymal Stem Cells for Potential Cytoplasm Replacement Therapy
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Cited by 10 •
2017
Age-related diseases are associated with multiple defects in components of the cytoplasm. Here, we present a protocol to prepare plasma membrane vesicles from bone marrow mesenchymal stem cells. This technique could potentially be used as a means of cytoplasm replacement therapy to ameliorate or even reverse age-associated phenotypes.
07:52
Detection of Antibodies That Neutralize the Cellular Uptake of Enzyme Replacement Therapies with a Cell-based Assay
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Cited by 4 •
2018
Here we present a cell-based flow cytometry method to detect neutralizing antibodies or other factors that interfere with the cellular uptake of enzyme replacement therapies in a human matrix, such as cerebral spinal fluid (CSF) or human serum.
FAQs
Resident stem and progenitor cells can proliferate, increasing the available cell population, and then differentiate into specialized cell types. This two-stage behavior links cell production with tissue-specific function. In biological studies, examining these processes helps explain how tissues maintain themselves and how their repair capacity may respond after injury.
Three outcomes are especially important: survival of the introduced or newly generated cells, integration with the surrounding tissue, and appropriate communication with neighboring cells. A replacement population may therefore be present without fully restoring function if it fails to connect or interact correctly. These criteria provide a framework for evaluating regenerative success.
Natural replacement relies on cells already residing in the tissue, particularly stem or progenitor cells, to proliferate and differentiate. Therapeutic approaches instead introduce donor or engineered cells into the affected tissue. This distinction separates regeneration driven by local biology from interventions designed to supplement or alter the tissue’s existing repair capacity.
Donor or engineered cells can provide an external source of replacement cells when the body cannot adequately regenerate a tissue. Their intended contribution depends on surviving in the target environment, integrating with surrounding structures, and communicating appropriately with neighboring cells. These requirements connect cell delivery to the possibility of restoring tissue function.
In biology, cell replacement supports investigations of tissue development, disease mechanisms, and recovery after injury. Researchers can use replacement systems to examine how specialized cells contribute to tissue function and how altered or damaged tissues respond when new cells are available. This makes the topic relevant to both basic biology and regenerative medicine.
Cell-based therapies are particularly relevant when a tissue cannot adequately replace lost, damaged, or dysfunctional cells on its own. The therapeutic goal is to restore function by introducing donor or engineered cells, but success depends on more than delivery alone. The cells must remain viable, integrate with the tissue, and communicate appropriately within it.