Journal
/
Biology
/
Feeder-Free Adaptation, Culture and Passaging of Human IPS Cells using Complete KnockOut Serum Replacement Feeder-Free Medium
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Feeder-Free Adaptation, Culture and Passaging of Human IPS Cells using Complete KnockOut Serum Replacement Feeder-Free Medium

Feeder-Free Adaptation, Culture and Passaging of Human IPS Cells using Complete KnockOut Serum Replacement Feeder-Free Medium

DOI:
10.3791/2236-v

•

08:29 min

•

July 15, 2010

•

,
1GIBCO,Life Technologies

Chapters

  • 00:04Title
  • 00:45Introduction
  • 01:33Preparing Geltrex-coated Culture Dishes
  • 02:59Adapting Human iPSCs
  • 05:20Passaging Human iPSCs
  • 07:36Representative Results
  • 08:07Conclusion

Summary

Automatic Translation

Automatic Translation

The following protocol provides instruction for adapting human induced Pluripotent Stem (iPS) Cells to feeder-free culture using complete KnockOut Serum Replacement Feeder-Free medium (KSR-FF). Once adapted, instructions for continual maintenance are also provided.

Tags

Feeder-Free AdaptationCulturePassagingHuman IPS CellsComplete KnockOut Serum ReplacementFeeder-Free MediumReprogrammedIPS CellsEmbryonic Stem CellsPluripotent CellsDrug DiscoveryCell TherapyBasic ResearchGIBCO Media And ReagentsKnockout DMEMKnockout Serum ReplacementIPS Cell CultureFeeder-free CultureKnockout SR Growth Factor CocktailTraditional Culture MethodsMouse Fibroblast Feeder LayersHuman Fibroblast Feeder LayersFeeder-conditioned MediumLabor-intensiveHard To ScaleUndifferentiated HiPS CellsUndefined Conditions

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/2236?article_type=v

Passaging HuES Human Embryonic Stem Cell-lines with Trypsin.
check_url/2236?article_type=v

Human ES cells: Starting Culture from Frozen Cells
check_url/2236?article_type=v

Predicting the Effectiveness of Population Replacement Strategy Using Mathematical Modeling
check_url/2236?article_type=v

Passaging Human Neural Stem Cells
check_url/2236?article_type=v

Culture and Maintenance of Human Embryonic Stem Cells
check_url/2236?article_type=v

Cryopreserving and Recovering of Human iPS Cells using Complete KnockOut Serum Replacement Feeder-Free Medium
check_url/2236?article_type=v

Generation of Human Cardiomyocytes: A Differentiation Protocol from Feeder-free Human Induced Pluripotent Stem Cells
check_url/2236?article_type=v

Isolation of Small Noncoding RNAs from Human Serum
check_url/2236?article_type=v

MicroRNA Expression Profiles of Human iPS Cells, Retinal Pigment Epithelium Derived From iPS, and Fetal Retinal Pigment Epithelium
check_url/2236?article_type=v

Preparation of Rat Serum Suitable for Mammalian Whole Embryo Culture

Read Article