Cell Tracking
Cell Tracking - Related Videos
07:59
Tracking Cells in GFP-transgenic Zebrafish Using the Photoconvertible PSmOrange System
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Cited by 2 •
2016
We established the photoconvertible PSmOrange system as a powerful, straight-forward and cost inexpensive tool for in vivo cell tracking in GFP transgenic backgrounds. This protocol describes its application in the zebrafish model system.
07:19
Cell Tracking Using Photoconvertible Proteins During Zebrafish Development
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Cited by 17 •
2012
Here, we present a method for the photoactivated switch of photoconvertible fluorescent proteins (PCFPs) in the living zebrafish embryo and further tracking of photoconverted protein at specific time points during development. This methodology allows monitoring of cell biological events underlying different developmental processes in a live vertebrate organism.
22:49
Optimized Staining and Proliferation Modeling Methods for Cell Division Monitoring using Cell Tracking Dyes
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Cited by 45 •
2012
Successful use of cell tracking dyes to monitor immune cell function and proliferation involves several critical steps. We describe methods for: 1) obtaining bright, uniform, reproducible label-ing with membrane dyes; 2) selecting fluorochromes and data acquisition conditions; and 3) choosing a model to quantify cell proliferation based on dye dilution.
09:14
Tracking Hypoxic Signaling within Encapsulated Cell Aggregates
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Cited by 10 •
2011
A method for photo-encapsulation of cells in a crosslinked PEG hydrogel is described. Hypoxic signaling within encapsulated murine insulinoma (MIN6) aggregates is tracked using a fluorescent marker system. This system allows serial examination of cells within a hydrogel scaffold and correlation of hypoxic signaling with changes in cell phenotype.
02:53
Tracking Bacterial Growth at Single-Cell Resolution in a Microfluidic System
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2025
Source: Cabeen, M. T., et al. Single-cell Microfluidic Analysis of Bacillus subtilis. J. Vis. Exp. (2018)The video demonstrates the use of a microfluidic device to observe bacterial growth at the single-cell level. It showcases how non-motile bacterial cells, confined within micro-scale trenches, are continuously nourished and imaged under a fluorescence microscope. The setup enables real-time visualization of cell division and lineage tracking across multiple generations.
FAQs
The analysis links observations believed to represent the same cell across successive image frames, then reconstructs that cell’s trajectory. From the resulting record, researchers can measure changes in location, movement, division, and interactions. This converts a sequence of microscopy images into time-resolved information about cellular behavior within a biological system.
Fluorescent dyes and genetically encoded markers provide signals that help identify cells in microscopy images over time. Their use supports the image-analysis step required to follow individual cells across frames. By making cellular identities traceable, these labels allow researchers to connect observations into trajectories rather than treating each image as an unrelated snapshot.
Tracking can quantify proliferation, differentiation, and cell-cell interactions in addition to changes in position. These measurements show whether cells divide, acquire different biological states, or influence one another over time. Such information helps researchers examine how groups of cells organize and respond to their surrounding biological environment.
A typical workflow begins by labeling cells with fluorescent dyes or genetically encoded markers. Researchers then acquire time-lapse microscopy images and apply image-analysis methods to associate the same cell across successive frames. The reconstructed trajectories can subsequently be examined for location, movement, division, differentiation, or interactions, depending on the biological question.
Cell Tracking can be applied to cell cultures, tissues, and developing organisms. These settings allow researchers to study cellular behavior at different levels of biological organization, from individual cells in a culture to coordinated activity within a tissue or developing system. The measurements can support investigations of migration, proliferation, differentiation, and cellular interactions.
By measuring cellular behavior over time, Cell Tracking helps connect individual cell actions with larger biological processes. It supports research in development, immunology, cancer biology, neuroscience, and tissue engineering, where location, movement, division, differentiation, or interactions may clarify how cells organize and respond to their environment.