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Tracking Cells in GFP-transgenic Zebrafish Using the Photoconvertible PSmOrange System
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Developmental Biology
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JoVE Journal Developmental Biology
Tracking Cells in GFP-transgenic Zebrafish Using the Photoconvertible PSmOrange System

Tracking Cells in GFP-transgenic Zebrafish Using the Photoconvertible PSmOrange System

DOI:
10.3791/53604-v

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07:59 min

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February 05, 2016

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, , ,
1Medical Faculty Mannheim, Department of Cell and Molecular Biology,Heidelberg University, 2COS and Nikon Imaging Center at the University of Heidelberg,Heidelberg University, 3Excellenzcluster CellNetworks,University of Heidelberg

Chapters

  • 00:05Title
  • 00:42PSmOrange Photoconversion
  • 04:16Analyzing the Fate of Photoconverted PSmOrange Protein Expressing Cells
  • 06:23Results: Cell Tracking in the Zebrafish Pineal Complex Using PSmOrange
  • 07:31Conclusion

Summary

Automatic Translation

Automatic Translation

We established the photoconvertible PSmOrange system as a powerful, straight-forward and cost inexpensive tool for in vivo cell tracking in GFP transgenic backgrounds. This protocol describes its application in the zebrafish model system.

Tags

Keywords: PSmOrangePhotoconversionGFPZebrafishCell TrackingConfocal MicroscopyDevelopmental BiologyRegenerationTumor ProgressionInvasion488 Nm Laser561 Nm LaserZ-stackRegion Of InterestPhotoactivation BleachingAcquisition StimulationPhotoconversion Protocol

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