Circadian Clock Modulation
Circadian Clock Modulation - Related Videos
07:42
Rapid Analysis of Circadian Phenotypes in Arabidopsis Protoplasts Transfected with a Luminescent Clock Reporter
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Cited by 18 •
2016
The circadian clock regulates about a third of the Arabidopsis transcriptome, but the percentage of genes that feed back into timekeeping remains unknown. Here we visualize a method to rapidly assess circadian phenotypes in any mutant line of Arabidopsis using luminescent imaging of a circadian reporter transiently expressed in protoplasts.
10:38
Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters
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Cited by 78 •
2012
Circadian clocks function within individual cells, i.e., they are cell-autonomous. Here, we describe methods for generating cell-autonomous clock models using non-invasive, luciferase-based real-time bioluminescence technology. Reporter cells provide tractable, functional model systems for studying circadian biology.
03:08
Embedding Drosophila Brain in a Matrix for Time-Lapse Imaging of the Circadian Clock Neurons
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2025
Source: Sabado, V., et al., Nagoshi, E. Single-cell Resolution Fluorescence Live Imaging of Drosophila Circadian Clocks in Larval Brain Culture. J. Vis. Exp. (2018). This video demonstrates the procedure to embed the Drosophila brain in a fibrin matrix, followed by time-lapse fluorescence imaging of circadian clock neurons to analyze rhythmic activity synchronized with light/dark cycles.
06:53
Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures
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Cited by 19 •
2016
Here, we describe settings to monitor in parallel circadian bioluminescence and the secretory activity of human islet cells and primary myotubes. For this, we employed lentiviral gene delivery of a luciferase core clock reporter, followed by in vitro synchronization and collection of outflow medium by continuous cell perifusion.
05:46
Recording and Analysis of Circadian Rhythms in Running-wheel Activity in Rodents
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Cited by 35 •
2013
Circadian rhythms in voluntary wheel-running activity in mammals are tightly coupled to the molecular oscillations of a master clock in the brain. As such, these daily rhythms in behavior can be used to study the influence of genetic, pharmacological, and environmental factors on the functioning of this circadian clock.
FAQs
Circadian clock modulation depends on communication between environmental timing cues and intracellular feedback. In mammals, light can reset the suprachiasmatic nucleus, a brain timing center, while clock proteins participate in transcriptional-translational feedback loops within cells. Together, these mechanisms help coordinate approximately 24-hour patterns with changes in hormone release, metabolism, sleep, and cellular activity.
These feedback loops provide a molecular mechanism for maintaining daily cycles inside cells. Clock proteins participate in recurring patterns of gene-related regulation, allowing cellular activity to follow approximately 24-hour timing. Because the same timing system influences hormone release, metabolism, and sleep, changes in its regulation can affect several physiological processes rather than producing an isolated cellular effect.
Light acts as an environmental cue that can reset the suprachiasmatic nucleus, whereas genetic changes can alter the molecular machinery that regulates daily cycles. The distinction separates an external influence on biological timing from an internal alteration of clock regulation. Both are important for understanding why biological rhythms may change, but they affect different levels of the timing system.
Researchers can examine changes in light exposure, work schedules associated with shift work, and genetic changes, then relate them to biological timing. Relevant outcomes include altered sleep, hormone release, metabolism, and cellular activity. Considering these factors together helps connect environmental conditions or molecular differences with broader changes in daily physiological and behavioral rhythms.
Circadian clock research is useful when sleep or metabolic function changes alongside disrupted biological timing. The overview identifies sleep disorders and metabolic disease as important applications because daily timing systems influence sleep and metabolism. Studying these relationships can help clarify how altered rhythms relate to disease-related patterns without treating timing disruption as separate from physiology.
Circadian clock modulation supports chronotherapy, an approach in which treatment schedules are aligned with daily changes in biological function. Since circadian timing influences hormone release, metabolism, sleep, and cellular activity, drug timing can be studied in relation to these changing states. This provides a biological basis for investigating whether treatment schedules should account for time-dependent physiology.