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Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures
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Genetics
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JoVE Journal Genetics
Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures

Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures

DOI:
10.3791/54673-v

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06:53 min

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November 11, 2016

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, , ,
1Department of Medical Specialties, Division of Endocrinology, Diabetes, Hypertension and Nutrition, Diabetes Center,University of Geneva Medical School, Institute of Genetics and Genomics in Geneva (iGE3), 2Population Epidemiology Unit (UEP), Community Medicine,Geneva University Hospital

Chapters

  • 00:05Title
  • 00:52Continuos Long-term Circadian Bioluminescence Recording, Performed in Parallel with the Assessment of Hormone Secretion in Living Human Primary Cells
  • 02:24Parallel Assessment of Circadian Bioluminescence Recording and Hormone Secretion Profiles in Synchronized Human Secretory Primary Cells
  • 04:37Results: Evaluation of the Clock Regulation of Basal IL-6 Secretion by Human Myotubes
  • 05:33Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we describe settings to monitor in parallel circadian bioluminescence and the secretory activity of human islet cells and primary myotubes. For this, we employed lentiviral gene delivery of a luciferase core clock reporter, followed by in vitro synchronization and collection of outflow medium by continuous cell perifusion.

Tags

Circadian ClockBioluminescenceHormone SecretionPrimary Cell CultureLentiviral TransductionSynchronizationCLOCKInsulin SecretionMyokine Secretion

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