Cisplatin
Cisplatin - Related Videos
13:25
Acute Kidney Injury Model Induced by Cisplatin in Adult Zebrafish
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Cited by 10 •
2021
This protocol describes the procedures to induce Acute Kidney Injury (AKI) in adult zebrafish using cisplatin as a nephrotoxic agent. We detailed the steps to evaluate the reproducibility of the technique and two techniques to analyze inflammation and cell death in the renal tissue, flow cytometry and TUNEL, respectively.
07:47
Custom-designed Laser-based Heating Apparatus for Triggered Release of Cisplatin from Thermosensitive Liposomes with Magnetic Resonance Image Guidance
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Cited by 17 •
2015
AN MRI-compatible custom-designed laser-based heating apparatus has been developed to provide local heating of subcutaneous tumors in order to activate release of agents from thermosensitive liposomes specifically at the tumor region.
04:20
Knockdown of FAM83A to Verify Its Role in Cervical Cancer Cell Growth and Cisplatin Sensitivity
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2024
Here, we show the procedures for FAM83A knockdown; the assays to detect its effects on proliferation, migration, and invasion of cervical cancer cells; and the sensitization of these cells to cisplatin. This study provides a promising target gene for cervical cancer and a reference for further drug research.
04:04
Endobronchial Ultrasound-guided Intratumoral Injection of Cisplatin for the Treatment of Isolated Mediastinal Recurrence of Lung Cancer
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Cited by 13 •
2017
The management of isolated recurrent lung cancer in a previously-irradiated field is challenging. Here, we describe an endobronchial ultrasound (EBUS)-guided cisplatin injection for the management of patients with localized lung cancer recurrence in a previously-radiated field.
06:14
Assessment of Mitochondrial Fission/Fusion Dynamics in Kidney Proximal Tubular Cells
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2025
This article describes the use of confocal microscopy and ImageJ to assess mitochondrial fission/fusion dynamics via Dendra2 photo-switching fluorescence.
FAQs
Activation occurs when chloride ligands are replaced by water. This change makes the platinum compound reactive enough to bind DNA bases, with guanine as a major target. The resulting intra- and interstrand crosslinks connect bases within one strand or across strands, creating lesions that alter DNA architecture and hinder its normal use.
Crosslink-induced distortion can block replication and transcription. Cells respond through DNA damage responses, including cell-cycle arrest and apoptosis. These outcomes help explain how cisplatin can suppress rapidly dividing cancer cells, while also providing a biological framework for studying how cells detect and respond to damage affecting their genetic material.
Its activity is not limited to cancer cells. Because cisplatin damages DNA in rapidly dividing cells, healthy tissues with dividing cells can also be affected. This lack of complete cellular selectivity helps explain toxicities such as kidney damage, hearing loss, and neuropathy, which are important biological and clinical considerations when evaluating treatment effects.
In cancer biology, cisplatin serves as a model for connecting a defined DNA lesion with a broader cellular response. Researchers use it to investigate DNA repair, drug resistance, and cellular stress responses, asking how cells handle the crosslinks and how those responses influence treatment effects. This makes the compound useful beyond its therapeutic role.
Key outcomes include DNA lesions, interference with replication and transcription, activation of DNA damage responses, cell-cycle arrest, and apoptosis. Examining these events helps relate the drug’s molecular action to changes in cell behavior. The same framework can also show how cancer cells and healthy tissues are affected by treatment.
Because cisplatin can damage healthy tissues as well as tumors, research must consider both anticancer activity and toxicity. Studies of DNA repair, drug resistance, and cellular stress responses provide biological context for strategies intended to improve targeted treatment. This work aims to make treatment effects more focused while accounting for kidney damage, hearing loss, and neuropathy.