Cold Plasma Sterilization
Cold Plasma Sterilization - Related Videos
06:36
Treating Surfaces with a Cold Atmospheric Pressure Plasma using the COST-Jet
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Cited by 6 •
2020
This protocol is presented to characterize the setup, handling, and application of the COST-Jet for the treatment of diverse surfaces such as solids and liquids.
02:20
Stimulating Nerve Stem Cell Differentiation Using Cold Atmospheric Plasma Treatment
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2025
This video presents a method for triggering differentiation in neural stem cells by utilizing cold atmospheric plasma. This generates UV radiation and reactive molecules to stimulate nerve cells to produce projections, indicating neuronal cell differentiation.
10:03
Investigating the Detrimental Effects of Low Pressure Plasma Sterilization on the Survival of Bacillus subtilis Spores Using Live Cell Microscopy
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Cited by 4 •
2017
This protocol illustrates the important consecutive steps required to assess the relevance of monitoring vitality parameter and DNA repair processes in reviving Bacillus subtilis spores after treatment with low pressure plasma by tracking fluorescence-labelled DNA repair proteins via time-resolved confocal microscopy and scanning electron microscopy.
02:45
Responses to Heat and Cold Stress
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2020
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes. When the environmental dynamics fall out of the optimal limit for a given species, changes in metabolism and functioning occur – and this is defined as stress. Plants respond to stress by initiating changes in gene expression - leading to adjustments in plant...
10:13
Working with Hot and Cold Sources
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2023
Source: Robert M. Rioux & Suprita Jharimune, Pennsylvania State University, University Park, PA Working with extreme temperatures, both high and low, is an integral part of many laboratory operations. For many, mentioning a laboratory instantly evokes the mental picture of a Bunsen burner. Bunsen burners and hot plates are used extensively in small and large operations in research laboratories and industries, thus making it necessary for all users to be aware of their safe handling...
FAQs
Microbial inactivation results from several damaging agents acting together. Reactive oxygen and nitrogen species attack cellular components, while charged particles and ultraviolet radiation contribute additional stress. These effects can disrupt membranes, alter or damage proteins, and injure nucleic acids. Because multiple cellular targets are affected, the process does not depend on a single type of microbial damage.
The low-temperature condition allows decontamination of heat-sensitive materials that could be damaged by conventional high-temperature processing. This expands its usefulness for selected instruments, surfaces, packaging, and biological materials. The temperature advantage also helps reduce thermal damage, while the plasma-generated reactive agents provide antimicrobial activity without relying on the intense heat used in conventional sterilization.
Effectiveness depends on plasma composition, exposure time, surface properties, and other treatment conditions. Changing the composition can alter the balance of reactive oxygen and nitrogen species, charged particles, and ultraviolet radiation reaching microorganisms. Exposure time and the characteristics of the treated surface also affect how consistently these agents contact and damage contaminants.
A laboratory workflow may apply the treatment to instruments, work surfaces, packaging, and selected biological materials, particularly when heat could cause unwanted damage. The material should be considered alongside its surface properties and the intended treatment conditions. Researchers must also account for exposure time and plasma composition because these variables influence the resulting decontamination outcome.
Applications extend to biomedical research, medical-device processing, food safety, and pathogen-control studies. These settings value the ability to address contamination while limiting thermal damage and chemical residues. The specific target may be an instrument, package, surface, or selected biological material, so the treatment conditions must be matched to the material and the decontamination objective.
Researchers should interpret the outcome in relation to the plasma composition, exposure time, surface properties, and overall treatment conditions rather than assuming identical performance across materials. These variables determine how effectively reactive species, charged particles, and ultraviolet radiation reach contaminants. Such evaluation is especially important when comparing laboratory surfaces, devices, packaging, or biological materials.