Colonoid Culture
Colonoid Culture - Related Videos
06:33
Establishment of Human Epithelial Enteroids and Colonoids from Whole Tissue and Biopsy
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Cited by 132 •
2015
We describe a method to establish human enteroids from small intestinal crypts and colonoids from colon crypts collected from both surgical tissue and biopsies. In this methodological article, we present the culture modalities that are essential for the successful growth and maintenance of human enteroids and colonoids.
02:04
Generation of a Human Colonoid Monolayer with Barrier Function to Study Host–Pathogen Interactions
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2026
Source: In, J. G.,et al. Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal Epithelium. J. Vis. Exp. (2019).This video demonstrates the procedure for culturing colon-derived epithelial cells on a collagen-coated Transwell system to generate a mucus-producing monolayer that models the intestinal barrier and enables host–pathogen interaction studies.
06:31
Studying the Epithelial Effects of Intestinal Inflammation In Vitro on Established Murine Colonoids
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Cited by 1 •
2023
We describe a protocol detailing the isolation of murine colonic crypts for the development of 3-dimensional colonoids. The established colonoids can then be terminally differentiated to reflect the cellular composition of the host epithelium prior to receiving an inflammatory challenge or being directed to establish an epithelial monolayer.
07:20
Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal Epithelium
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Cited by 45 •
2019
Here, we present a protocol to culture human enteroid or colonoid monolayers that have intact barrier function to study host epithelial-microbiota interactions at the cellular and biochemical level.
08:45
Method for Culture of Early Chick Embryos ex vivo (New Culture)
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Cited by 9 •
2008
This video demonstrates New culture, a method by which chick embryos are cultured outside the egg for up to 24 hr. This method enables one to study early development (primitive streak to 14 som.), a period corresponding to E7-9 in mouse. Applications of this technique include electroporation, in situ hybridization and immunohistochemistry.
FAQs
An extracellular matrix helps organize the cells in a three-dimensional setting, while defined growth factors provide signals that support stem-cell maintenance and proliferation. Together, these conditions allow the cells to self-organize rather than simply remain as an undifferentiated population. The resulting structure makes colonoid culture useful for examining controlled aspects of colon epithelial biology.
Defined growth factors establish the culture conditions needed to preserve intestinal stem-cell characteristics and promote cell expansion. Their role is therefore functional, not merely nutritional: they help maintain the population that drives continued tissue formation. Using defined signals gives researchers a controlled way to investigate how colon epithelial cells renew and organize in vitro.
These features provide an architectural readout of how cells arrange into tissue-like compartments. Their presence lets researchers examine relationships between colon epithelial organization and processes such as renewal, rather than studying cell behavior without structural context. Because the structures arise within the culture, investigators can observe aspects of colon tissue organization under defined experimental conditions.
Cells are obtained from healthy or patient-derived intestinal tissue, embedded in an extracellular matrix, and supplied with defined growth factors. The culture is then maintained under conditions that support stem-cell maintenance, proliferation, and self-organization. This workflow produces crypt- and lumen-like organization that can be examined as a model of colon epithelial biology.
Healthy tissue can support studies of normal epithelial biology, whereas patient-derived tissue can preserve a disease-relevant context for modeling. Comparing these sources helps connect general mechanisms with individual disease biology. This distinction is especially valuable when investigating inflammation, infection, cancer, or potential treatment responses in a controlled culture system.
Colonoid culture supports questions about epithelial renewal and how colon tissue responds to disease-related challenges. Researchers can apply the system to investigate host–microbe interactions, inflammation, infection, and cancer, while retaining a controlled in vitro setting. Its three-dimensional organization links cellular behavior with tissue-like structure, helping biological studies move beyond isolated observations from individual cells.
Colonoids can be used to assess how colon-derived tissue responds to candidate treatments in a controlled setting. Drug-response studies can be performed with cultures originating from healthy or patient-derived tissue, allowing therapeutic testing to be connected with either baseline epithelial biology or disease-associated biology. This makes the system relevant to both general pharmacology research and personalized disease modeling.