Colony Patching
Colony Patching - Related Videos
09:43
Patch Clamp Electrophysiology
0 Views •
2023
Neuron cell membranes are populated with ion channels that control the movement of charge into and out of the cell, thereby regulating neuron firing. One extremely useful technique for investigating the biophysical properties of these channels is called patch clamp recording. In this method, neuroscientists place a polished glass micropipette against a cell and apply suction to form a high resistance seal. This process isolates a small “patch” of membrane that contains one or more ion channels.
06:44
Keratinocyte Colony Formation Assay: An In Vitro Assay to Assess the Ability of Keratinocytes to Grow into Colonies
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2023
In this video, we perform a clonogenic assay of keratinocytes harvested from adult mice to determine the ability of individual cells to proliferate and form colonies. This keratinocyte harvesting technique is useful for studying other downstream aspects of cutaneous chemical carcinogenesis and tumor promotion.
01:02
Long-patch Base Excision Repair
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2020
Since the discovery of the two BER pathways, there has been a debate about how a cell chooses one pathway over the other and the factors determining this selection. Numerous in vitro experiments have pointed out multiple determinants for the sub-pathway selection. These are: Lesion type: Depending on the type of base damage, a specific DNA glycosylase - mono or bifunctional, is recruited to the damaged site. While the sequential action of a monofunctional glycosylase favors long patch repair...
06:02
Isolation of Lymphocytes from a Mouse Peyer's Patch
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2025
In this video, we demonstrate the isolation of lymphocytes from a harvested mouse Peyer's patch. The isolated lymphocytes can be used for further analysis.
09:04
Yeast Colony Embedding Method
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Cited by 7 •
2011
A method for embedding yeast colonies allowing sectioning for light and electron microscopy. This protocol allows determination of the distribution of sporulated cells and pseudohyphal cells within colonies providing a new tool toward understanding the organization of cell types within a fungal community.
FAQs
Preserving each colony’s position creates a spatial map between the source plate and every test plate. Researchers can therefore match growth or failure on a test condition to the corresponding source colony without mixing strains. This positional relationship makes comparisons more reliable when screening many colonies for differences in phenotype, such as resistance or nutrient dependence.
A single source plate can provide material for comparison across several defined conditions. Because the relative positions remain consistent, the same colony can be evaluated on selective media, under temperature stress, or with nutrient limitation. Differences among these plates help distinguish general growth capacity from a phenotype that appears only under a particular condition.
Selective conditions expose phenotypes that may not be evident during routine growth. A colony that grows on one test plate but not another may show antibiotic resistance, auxotrophy, or conditional viability, depending on the imposed condition. Comparing these patterns links an observable growth response to the treatment or nutritional environment used in the experiment.
The workflow begins with colonies on a source plate and a sterile velvet surface, replica-plating tool, or pipette. Cells from the colonies are transferred while their relative positions are maintained, then placed onto one or more test plates. The transferred cells grow under defined conditions, and researchers compare the resulting patterns with the source arrangement.
Colony Patching is useful when researchers need to examine many microbial colonies for a growth phenotype associated with genotype. Screening can identify mutants that fail under a defined condition, strains with antibiotic resistance, or colonies requiring particular nutrients. The method also supports confirmation of transformed cells by comparing growth on an appropriate test condition.
The technique provides a direct way to compare how individual strains respond to controlled growth conditions. Matching each test-plate result to its source colony allows researchers to characterize phenotypic differences across a population and relate those differences to genetic screening or treatment conditions. This makes the approach valuable for identifying mutant behavior and conditional growth patterns.