Colony Sectioning
Colony Sectioning - Related Videos
08:52
Paraffin Embedding and Thin Sectioning of Microbial Colony Biofilms for Microscopic Analysis
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Cited by 23 •
2018
We describe fixation, paraffin embedding, and thin sectioning techniques for microbial colony biofilms. In prepared samples, biofilm substructure and reporter expression patterns can be visualized by microscopy.
09:04
Yeast Colony Embedding Method
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Cited by 7 •
2011
A method for embedding yeast colonies allowing sectioning for light and electron microscopy. This protocol allows determination of the distribution of sporulated cells and pseudohyphal cells within colonies providing a new tool toward understanding the organization of cell types within a fungal community.
06:44
Keratinocyte Colony Formation Assay: An In Vitro Assay to Assess the Ability of Keratinocytes to Grow into Colonies
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2023
In this video, we perform a clonogenic assay of keratinocytes harvested from adult mice to determine the ability of individual cells to proliferate and form colonies. This keratinocyte harvesting technique is useful for studying other downstream aspects of cutaneous chemical carcinogenesis and tumor promotion.
01:50
Cross-Sectional Research
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2020
In cross-sectional research, a researcher compares multiple segments of the population at the same time. If they were interested in people's dietary habits, the researcher might directly compare different groups of people by age. Instead of following a group of people for 20 years to see how their dietary habits changed from decade to decade, the researcher would study a group of 20-year-old individuals and compare them to a group of 30-year-old individuals and a group of 40-year-old...
07:47
ScanLag: High-throughput Quantification of Colony Growth and Lag Time
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Cited by 66 •
2014
ScanLag is a high-throughput method for measuring the delay in growth, namely lag time, as well as the growth rate of colonies for thousands of cells in parallel. Screening using ScanLag enables the discrimination between long lag-time and slow growth at the level of a single variant.
FAQs
Spatial arrangement allows researchers to compare regions according to their original position within the colony rather than treating the colony as uniform. This distinction can reveal localized differences in morphology, growth, cell behavior, or genetic characteristics. Maintaining that context strengthens comparisons between sections and helps connect observed variation with local conditions influencing colony development.
Controlled handling limits accidental movement of material between defined regions and helps preserve the distinctions being studied. Sterile technique is important because cross-contamination could make a section appear to contain traits from another region, weakening conclusions about localized variation. Careful separation therefore improves the reliability of independent examination or subsequent transfer.
Colony sectioning can expose differences in morphology, growth, cell behavior, or genetic characteristics among regions of the same colony. These comparisons are useful when a colony contains distinguishable areas or when local conditions may affect development. Examining sections independently helps determine whether an observed trait is broadly distributed or concentrated in a particular region.
A researcher first identifies the regions to compare, then uses a sterile tool to divide the colony into defined sections while keeping their spatial relationships clear. Each portion is handled separately to limit cross-contamination. The resulting sections can be subcultured or examined independently, allowing observations or transfers to remain linked to their original colony locations.
Each section can be examined independently for localized features or transferred into a separate subculture. Independent handling makes it possible to compare regions directly and to retain selected portions for further study. The resulting observations can support analysis of morphology, growth, cell behavior, or genetic characteristics without combining information from different colony areas.
The technique is useful for clonal isolation, phenotypic screening, strain selection, and studying how local conditions influence colony development. By separating regions and retaining their identities, researchers can identify portions with relevant characteristics and evaluate them independently. This makes the approach valuable when spatially localized differences matter to biological interpretation or experimental selection.