Confocal Scanning Microscopy
Confocal Scanning Microscopy - Related Videos
14:10
Video-rate Scanning Confocal Microscopy and Microendoscopy
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Cited by 14 •
2011
The complete construction of a custom, real-time confocal scanning imaging system is described. This system, which can be readily used for video-rate microscopy and microendoscopy, allows for an array of imaging geometries and applications not accessible using standard commercial confocal systems, at a fraction of the cost.
09:44
Oral Biofilm Analysis of Palatal Expanders by Fluorescence In-Situ Hybridization and Confocal Laser Scanning Microscopy
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Cited by 20 •
2011
We present a protocol for structural and compositional analysis of natural oral biofilm from orthodontic appliances with in situ hybridization (FISH) and confocal laser scanning microscopy (CLSM). Oral biofilm samples were collected from palatal expanders, scraping acrylic-resin flakes off their surface and referring them for molecular processing.
02:07
Imaging Tumor Cell Migration in Glioblastoma Tissue Using Time-Lapse Scanning Confocal Microscopy
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2025
Source: Parker, J. J., et al. A Human Glioblastoma Organotypic Slice Culture Model for Study of Tumor Cell Migration and Patient-specific Effects of Anti-Invasive Drugs. J. Vis. Exp. (2017)This video showcases the imaging of glioblastoma tissue slices expressing green fluorescent protein (GFP) via confocal microscopy. High-resolution images are captured at consistent intervals to track tumor cell movement and direction.
09:20
Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells
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Cited by 11 •
2020
Presented here is the protocol of the Conpokal technique, which combines confocal and atomic force microscopy into a single instrument platform. Conpokal provides same cell, same region, near simultaneous confocal imaging and mechanical characterization of live biological samples.
10:48
Imaging Biological Samples with Optical and Confocal Microscopy
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2023
Source: Peiman Shahbeigi-Roodposhti and Sina Shahbazmohamadi, Biomedical Engineering Department, University of Connecticut, Storrs, Connecticut Optical microscopes have been around for centuries, and while they reached their theoretical limitation of resolution decades ago, new equipment and techniques, such as confocal and digital image processing, have created new niches within the field of optical imaging. The best optical microscopes will typically have a resolution down to 200 nm in ideal...
FAQs
The spatial pinhole excludes emitted or reflected light that does not originate from the focal plane. By limiting detected signal to light associated with the focused point, it reduces interference from structures located above or below that plane. This selective collection improves image contrast and helps reveal fine cellular or tissue features more clearly.
Point-by-point laser scanning provides localized illumination and measurement across the specimen. The system records signal from each focused position, preserving spatial information as the scan progresses. Computer software combines these measurements into an image, allowing researchers to examine labeled structures with high spatial detail rather than relying on an undifferentiated signal from the entire field.
The microscope collects information from focused planes at different depths within a specimen. Each depth can be represented as an optical section, and software reconstructs the collected sections into a three-dimensional image. This organization of data helps investigators assess how cells, proteins, or tissue features are arranged relative to one another through the specimen.
Fluorescent labeling generates detectable signal from selected cells, proteins, or subcellular structures, while the confocal system limits contributions from outside the focal plane. The resulting images can show where labeled targets occur within biological material. This makes the technique useful for examining localization and organization while retaining depth information across cells or tissues.
A specimen is prepared with fluorescent labeling or another detectable signal, placed for imaging, and scanned with a focused laser point by point. The system collects emitted or reflected light while the pinhole rejects out-of-focus contributions. Software then assembles the measurements into optical sections or a three-dimensional representation for analysis.
Researchers can use confocal scanning microscopy when they need improved contrast, spatial resolution, and depth information in biological specimens. It is particularly relevant for investigating cell organization, tissue architecture, and the location of proteins or other labeled structures. The ability to examine multiple focal planes also supports analysis of complex specimens rather than isolated surface features.
The technique can reveal the spatial distribution of fluorescently labeled subcellular structures within cells. Optical sections help show whether features occupy different depths or share particular spatial relationships, while three-dimensional reconstruction supports assessment of their overall arrangement. These observations contribute to studies of cell organization and protein localization, and can also support examination of biological processes over time.