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1Department of Orthodontics and Maxillofacial Orthopedics,Medical University of Graz, 2Institute of Hygiene, Microbiology and Environmental Medicine,Medical University of Graz, 3Department of Prosthodontics, Restorative Dentistry, Periodontology and Implantology,Medical University of Graz, 4Institute of Plant Sciences,Karl-Franzens-University Graz
Chapters
- 00:05Title
- 01:51Specimen Collection and Biofilm Fixation
- 03:38Dehydration of Fixed Samples
- 04:34In-situ Hybridization and Imaging
- 07:21Results: Biofilm Images
- 08:56Conclusion
We present a protocol for structural and compositional analysis of natural oral biofilm from orthodontic appliances with in situ hybridization (FISH) and confocal laser scanning microscopy (CLSM). Oral biofilm samples were collected from palatal expanders, scraping acrylic-resin flakes off their surface and referring them for molecular processing.
