Copper Grids
Copper Grids - Related Videos
06:52
Positron Emission Tomography Using 64-Copper as a Tracer for the Study of Copper-Related Disorders
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Cited by 6 •
2023
The present protocol describes how to perform 64Cu PET/CT and PET/MRI imaging in humans to study copper-related disorders, such as Wilson disease, and the treatment effect on copper metabolism.
06:45
A Caenorhabditis elegans Nutritional-status Based Copper Aversion Assay
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Cited by 3 •
2017
Here, we present a Caenorhabditis elegans-specific assay designed to evaluate changes in copper aversion behavior and the ability to locate a common food source, as the organism progresses from a well-fed to starved nutritional state.
12:04
Exfoliation of Egyptian Blue and Han Blue, Two Alkali Earth Copper Silicate-based Pigments
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Cited by 34 •
2014
The preparation and exfoliation of CaCuSi4O10 and BaCuSi4O10 are described. Upon stirring in hot water, CaCuSi4O10 spontaneously exfoliates into monolayers, whereas BaCuSi4O10 requires ultrasonication in organic solvents. Near infrared (NIR) imaging illustrates the NIR emission properties of these materials, and aqueous dispersions of these nanomaterials are useful for solution processing.
08:01
Visualizing Proteins and Macromolecular Complexes by Negative Stain EM: from Grid Preparation to Image Acquisition
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Cited by 137 •
2011
Visualizing protein samples by negative stain electron microscopy (EM) has become a popular structural analysis method. It is useful for quantitative structural analysis, such as calculating a 3D reconstruction of the molecules being studied, and also for qualitative examination of the quality of protein preparations. In this article we present detailed protocols for preparing the EM grids, staining the sample and visualizing the sample in an electron microscope. Novice users can follow these...
05:57
Fabrication of Antibacterial Graphene Oxide/Copper Nanocomposites
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2024
Herein, we introduce graphene oxide/copper (GO/Cu) nanocomposites as an antibacterial nanomaterial. The antibacterial effectiveness of the GO/Cu nanocomposites was evaluated against both antibiotic-resistant gram-positive and gram-negative bacteria.
FAQs
Copper helps dissipate electrical charge that can accumulate during electron-beam exposure. This charge management supports a more stable imaging platform while the specimen remains positioned for observation. In biological TEM, the conductive metal therefore contributes to reliable visualization of cellular ultrastructure, organelles, macromolecular assemblies, and pathogens rather than serving only as a physical support.
Biological samples are placed on a carbon-coated surface before TEM imaging. The coating provides the immediate support on which ultrathin sections, isolated organelles, or negatively stained viruses can be positioned, while the copper grid supplies the underlying conductive mesh. Together, these components hold the specimen in the beam path and support imaging through the open areas.
The mesh openings permit the electron beam to pass through regions containing the specimen, while the grid provides structural support around those openings. This arrangement allows investigators to examine material distributed across the grid without requiring a solid support beneath every region. The resulting images can reveal cellular organization and other structures at high resolution.
The supported specimen types include ultrathin cell sections, isolated organelles, and negatively stained viruses. Each preparation places biological material on a carbon-coated grid so the electron beam can interact with and pass through the relevant regions. This range makes the same grid format useful for examining whole-cell organization, subcellular components, and pathogen structure.
A biological specimen is prepared in a form suitable for electron transmission, placed on a carbon-coated grid, and positioned so the electron beam can pass through the specimen and nearby mesh openings. The grid then supports imaging in the TEM. The workflow accommodates ultrathin sections, isolated organelles, and negatively stained viruses.
They are useful when researchers need high-resolution information about structures too small for light microscopy. Applications include studying cellular organization, examining infection-related pathogens, and assessing structural changes caused by disease or experimental treatments. By supporting imaging of sections, organelles, and viruses, the grids connect specimen preparation with detailed ultrastructural interpretation.