Culture Supernatant
Culture Supernatant - Related Videos
01:49
An Assay To Evaluate the Anticancer Effects of Lactobacillus Cell-Free Supernatant
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2025
This video demonstrates an in vitro assay to study the anticancer effects of Lactobacillus cell-free supernatant (LCFS). Upon exposing colorectal cancer spheroids to varying doses of LCFS, the interaction with the bacterial metabolites in LCFS induces apoptosis in the cancer cells, disrupting the spheroid structure and leading to cell death in a dose-dependent manner.
06:07
Evaluating Cell Death Using Cell-Free Supernatant of Probiotics in Three-Dimensional Spheroid Cultures of Colorectal Cancer Cells
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Cited by 15 •
2020
Here methods are presented to understand anti-cancer effects of Lactobacillus cell-free supernatant (LCFS). Colorectal cancer cell lines show cell deaths when treated with LCFS in 3D cultures. The process of generating spheroids can be optimized depending on the scaffold and the analysis methods presented are useful for evaluating the involved signaling pathways.
08:36
Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction
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Cited by 7 •
2018
Real-time quantitative polymerase chain reaction analysis combined with reverse transcription (RT-qPCR) has been widely used to measure the level of RNA virus infections. Here we present a direct RT-qPCR assay, which does not require an RNA purification step, developed for the quantification of several RNA viruses, including dengue virus.
03:40
Heparin Affinity Chromatography-Based Baculovirus Purification: A Technique to Isolate Baculovirus From Insect Cell Supernatant
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2025
In this video, we demonstrate the technique to isolate baculovirus from an insect cell supernatant using heparin affinity chromatography. The isolation is facilitated by the affinity between the heparin in the column to the baculovirus envelope glycoprotein.
02:51
Culturing Non-typeable Haemophilus influenzae
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2025
Source: Venuprasad, K. et. al., Intra-tracheal Administration of Haemophilus influenzae in Mouse Models to Study Airway Inflammation. J. Vis. Exp. (2016)This video demonstrates the culturing of non-typeable Haemophilus influenzae and the preparation of a dose-standardized inoculum for downstream colonization or infection studies.
FAQs
The composition reflects both cellular output and the surrounding growth environment. Cells may release or secrete soluble proteins, metabolites, signaling molecules, and other extracellular products into the medium, while dissolved components already present in the medium remain. Consequently, findings from Culture Supernatant can represent cell activity together with medium-derived material, so interpretation must consider both sources.
Because sampling the liquid does not require direct analysis of the cell population itself, it can provide a non-destructive view of behavior over time. Examination can focus on substances associated with cellular communication, metabolism, and responses to experimental conditions, while preserving the value of the culture for continued observation.
Separation is important because the liquid fraction is intended to retain soluble extracellular information while removing cells and debris. Centrifugation or filtration helps distinguish released or secreted substances from material associated with intact cells or particulate debris. This distinction makes subsequent analysis more focused on what the culture contributes to its surrounding medium.
After incubation, the culture is processed to separate liquid from cells and debris. Centrifugation or filtration may be used, depending on how the sample is handled, and the recovered liquid is retained for analysis. The essential procedural goal is to obtain a clarified liquid fraction without losing soluble proteins, metabolites, signaling molecules, or other extracellular products of interest.
It can support assays focused on secreted factors, immune mediators, microbial products, and potential therapeutic compounds. These measurements help connect extracellular contents with cellular activity or microbial behavior, making the sample useful when the research question concerns products released into the surrounding medium rather than only the cells themselves.
Researchers can examine the supernatant to assess how cellular activity, communication, or metabolism is reflected outside the cells under different experimental conditions. In biology, this provides a complementary perspective to observing cells directly and can help identify extracellular changes associated with the condition being studied.