Culture System
Culture System - Related Videos
08:45
Method for Culture of Early Chick Embryos ex vivo (New Culture)
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Cited by 9 •
2008
This video demonstrates New culture, a method by which chick embryos are cultured outside the egg for up to 24 hr. This method enables one to study early development (primitive streak to 14 som.), a period corresponding to E7-9 in mouse. Applications of this technique include electroporation, in situ hybridization and immunohistochemistry.
10:41
Culture and Co-Culture of Mouse Ovaries and Ovarian Follicles
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Cited by 16 •
2015
This protocol describes the primary culture/co-culture of mouse ovarian tissue, using ovaries from neonatal mice and individual ovarian follicles from prepubertal mice. The culture techniques support development in a highly physiological manner, allowing investigation of the effect of extrinsic agents on the ovary, and of interactions between ovarian follicles.
09:28
Culturing Lymphocytes in Simulated Microgravity Using a Rotary Cell Culture System
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Cited by 2 •
2022
This is a step-by-step guide for using a commercially available rotary cell culture system to culture lymphocytes in simulated microgravity using specialized disposable culture vessels. This culturing method may be applied to any suspension-type cell culture.
08:47
Culturing Mouse Cardiac Valves in the Miniature Tissue Culture System
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Cited by 10 •
2015
Here, we present an ex vivo flow model in which murine cardiac valves can be cultured allowing the study of the biology of the valve.
09:41
Establishment of an Extracellular Acidic pH Culture System
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Cited by 13 •
2017
The acidic tumor microenvironment plays critical roles in tumor progression. To assess the effects of acidic extracellular pH on cancer cells in vitro, we established simple acidic culture systems.
FAQs
These variables jointly determine whether cells, tissues, or microorganisms remain viable, divide, and produce measurable responses. Nutrients support biological activity, while temperature, pH, and gas conditions create the chemical and physical surroundings needed for growth. Because changing one condition can alter the overall response, researchers control them together when comparing development, disease mechanisms, or treatment effects.
The culture format determines how biological material is maintained and studied. Liquid media provide a fluid environment, whereas solid substrates offer a surface, and supporting matrices provide additional structural context. Researchers select among these formats according to the organism or tissue being examined and the research goal, such as studying growth, development, cell function, or tissue engineering.
Sterility helps prevent unwanted biological material from altering the culture conditions or competing with the material under study. This is especially important when researchers measure growth, cellular responses, or microorganism behavior, because unintended changes could make results difficult to interpret. Maintaining a controlled, sterile environment therefore supports clearer links between the experimental conditions and observed outcomes.
Researchers first match the culture format and supporting environment to the biological material and experimental objective. They then provide suitable nutrients, temperature, pH, gas conditions, and sterility, using liquid media, solid substrates, specialized vessels, or supporting matrices as appropriate. Once maintained under those conditions, the system can be monitored for growth, development, function, or other measurable responses.
A culture system is useful when investigators need to observe biological material under controlled conditions while changing a relevant treatment or environmental factor. Such setups can support studies of disease mechanisms and responses to drugs, allowing researchers to examine measurable changes in growth, cell function, or other biological behavior without relying only on observations from the natural environment.
These systems provide controlled conditions for maintaining and studying biological materials while researchers examine growth, development, and function. In biotechnology, they support investigations involving microorganisms or cells; in tissue engineering and regenerative research, supporting matrices and specialized vessels can help create an appropriate study environment. The resulting observations inform how biological materials respond under defined laboratory conditions.