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JoVE Journal
Developmental Biology

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Culture and Co-Culture of Mouse Ovaries and Ovarian Follicles
 

Culture and Co-Culture of Mouse Ovaries and Ovarian Follicles

Article DOI: 10.3791/52458
March 17th, 2015

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Summary March 17th, 2015

This protocol describes the primary culture/co-culture of mouse ovarian tissue, using ovaries from neonatal mice and individual ovarian follicles from prepubertal mice. The culture techniques support development in a highly physiological manner, allowing investigation of the effect of extrinsic agents on the ovary, and of interactions between ovarian follicles.

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