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JoVE Journal
Developmental Biology

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Culture and Co-Culture of Mouse Ovaries and Ovarian Follicles
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Culture and Co-Culture of Mouse Ovaries and Ovarian Follicles

Article DOI: 10.3791/52458
March 17th, 2015 •
1, 1, 1, 2, 1
1Centre for Integrative Physiology, University of Edinburgh, 2MRC Centre for Reproductive Health, University of Edinburgh

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March 17th, 2015

This protocol describes the primary culture/co-culture of mouse ovarian tissue, using ovaries from neonatal mice and individual ovarian follicles from prepubertal mice. The culture techniques support development in a highly physiological manner, allowing investigation of the effect of extrinsic agents on the ovary, and of interactions between ovarian follicles.

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