Cyclin A2 Function
Cyclin A2 Function - Related Videos
10:54
Studying Proteolysis of Cyclin B at the Single Cell Level in Whole Cell Populations
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Cited by 2 •
2012
Metaphase to anaphase transition is triggered through anaphase-promoting complex (APC/C)-dependent ubiquitination and subsequent destruction of cyclin B. Here, we established a system which, following pulse-chase labeling, allows monitoring cyclin B proteolysis in entire cell populations and facilitates the detection of interference by the mitotic checkpoint.
01:45
Positive Regulator Molecules
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2019
To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated. Internal regulatory checkpoints ensure that a cell’s size, energy reserves, and DNA quality and completeness are sufficient to advance through the cell cycle. At these checkpoints, positive and negative regulators promote or inhibit a cell’s continuation through the cell cycle. Positive regulators include two protein groups that allow cells to pass through regulatory...
07:00
A Method to Study the C924T Polymorphism of the Thromboxane A2 Receptor Gene
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2019
In the present study, we describe a methodology to analyze the C924T genotype. The protocol consists of three phases: DNA extraction, amplification by polymerase chain reaction (PCR), and analysis of the restriction fragment length polymorphism (RFLP) on agarose gel.
12:26
Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
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Cited by 10 •
2018
Cyclin-dependent kinase 1 (Cdk1) is activated in the G2 phase of the cell cycle and regulates many cellular pathways. Here, we present a protocol for an in vitro kinase assay with Cdk1, which allows the identification of Cdk1-specific phosphorylation sites for establishing cellular targets of this important kinase.
02:45
Functional Groups
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2020
Functional groups are a group of atoms with characteristic properties, which when linked to the carbon skeleton of a molecule, alter the properties of that molecule. For example, the presence of certain functional groups on a molecule will make them hydrophilic, whereas others will make them hydrophobic. These functional groups are an indispensable part of organic chemistry and important components of biological molecules, such as carbohydrates, proteins, lipids, and nucleic acids. Each...
FAQs
Cyclin A2 supports distinct cell-cycle requirements through its associations with CDK2 and CDK1. During S phase, its activity relates to DNA replication, while later activity contributes to the transition into mitosis. This stage-dependent partnership allows one regulatory protein to coordinate progression between major cell-cycle phases rather than acting as a uniform signal throughout the cycle.
CDK2 and CDK1 provide the kinase partners through which Cyclin A2 influences cell-cycle events. Their activation enables phosphorylation of target proteins, connecting Cyclin A2 to DNA replication, centrosome activity, and entry into mitosis. Examining these partnerships helps distinguish how Cyclin A2 contributes to different cellular processes and clarifies the mechanisms linking protein regulation with cell division.
Degradation at the appropriate time helps cells move from one cell-cycle stage to the next. If Cyclin A2 remains present beyond the interval in which its activity is needed, the normal timing of progression could be disrupted; if it is removed prematurely, required activities may not be supported. Its controlled disappearance therefore complements its accumulation during S phase.
Cyclin A2 function extends to centrosome activity and the transition into mitosis, in addition to DNA replication. These connections place the protein at several points where cells prepare for and execute division. Studying all three outcomes gives a broader view of how cell-cycle regulation coordinates replication with the physical and temporal events required for successful cell division.
Because Cyclin A2 helps coordinate replication and cell division, its regulation is relevant to how cells proliferate during development. Investigating when it accumulates, which kinase partners it engages, and when it is degraded can clarify how cells progress through the cycle. This provides biological context for understanding normal growth as well as altered patterns of proliferation.
Abnormal regulation of Cyclin A2 can support uncontrolled cell division, making it relevant to cancer biology. Its role also creates a basis for evaluating therapies that target the cell cycle, because such studies can consider whether treatment affects the regulatory processes governing replication and mitotic entry. The protein therefore links fundamental cell-cycle research with disease-focused investigation.