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Studying Proteolysis of Cyclin B at the Single Cell Level in Whole Cell Populations

Article DOI: 10.3791/4239
September 17th, 2012 •
1, 1, 1, 1, 1
1Department of Hematology, Oncology and Stem Cell Transplantation, University Medical Center Freiburg

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Automatic Translation
September 17th, 2012

Metaphase to anaphase transition is triggered through anaphase-promoting complex (APC/C)-dependent ubiquitination and subsequent destruction of cyclin B. Here, we established a system which, following pulse-chase labeling, allows monitoring cyclin B proteolysis in entire cell populations and facilitates the detection of interference by the mitotic checkpoint.

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