Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / A Method to Study the C924T Polymorphism of the…
JoVE Journal
Genetics

This content is Open Access.

JoVE Journal Genetics
A Method to Study the C924T Polymorphism of the Thromboxane A2 Receptor Gene
A Universal Protocol for Large-scale gRNA Library Production from any DNA Source
Journal (Genetics)
A Universal Protocol for Large-scale gRNA Library Production from any DNA Source
CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis
Journal (Genetics)
CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis
 

A Method to Study the C924T Polymorphism of the Thromboxane A2 Receptor Gene

Article DOI: 10.3791/57289-v • 07:00 min • April 1st, 2019
April 1st, 2019 •
1, 1, 2, 2, 2, 1, 3, 1, 2, 4, 1,2
1SS Annunziata University Hospital, Unit of Clinical Molecular Biology and Predictive Medicine, University of Chieti, 2Department of Medical, Oral and Biotechnological Sciences, University of Chieti, 3Department of Biotechnological and Applied Clinical Sciences, University of L'Aquila, 4CNR - Institute of Molecular Genetics, Section of Chieti

Chapters

Summary
Automatic Translation

In the present study, we describe a methodology to analyze the C924T genotype. The protocol consists of three phases: DNA extraction, amplification by polymerase chain reaction (PCR), and analysis of the restriction fragment length polymorphism (RFLP) on agarose gel.

Tags

C924T Polymorphism Thromboxane A2 Receptor Gene Method Study Thrombosis Hemostasis Atherothrombotic Processes High-risk Patients Aspirin Inexpensive Technique Simple To Use Convenient High Efficiency Rapid Identification Polymorphisms Personalized Medicine Specific Drugs Pharmacological Individual Response Tris EDTA Buffer Electrophoresis Buffer Gel Loading Dye 1X TBE Buffer Agarose
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter