Deciduous Teeth

Deciduous teeth, also called primary or baby teeth, are the first set of teeth to develop and erupt in humans, supporting early feeding, speech, and jaw development. They form within the embryonic dental lamina, mineralize during development, and emerge through the gums as the roots and surrounding tissues mature; later, resorption of their roots allows permanent teeth to replace them. Studying deciduous teeth helps biologists understand craniofacial development, tooth eruption, and the coordinated transition from primary to permanent dentition. Their condition also provides important information about childhood oral health, nutrition, and developmental disturbances.

Deciduous Teeth - Related Videos

Research

JoVE Journal - Bioengineering

Isolation, Culture, and Characterization of Dental Pulp Stem Cells from Human Deciduous and Permanent Teeth

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Cited by 6 •

2024

This protocol emphasizes the extraction, culture, and preservation of multipotent stem cells from dental pulp through enzymatic digestion. Additionally, it demonstrates their potential to differentiate into osteoblasts, adipocytes, and chondrocytes, highlighting the importance of precision and consistency in the process.

Single-Cell Sorting of Immunophenotyped Mesenchymal Stem Cells from Human Exfoliated Deciduous Teeth

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Cited by 2 •

2023

This protocol describes the use of fluorescence-activated cell sorting of human mesenchymal stem cells using the single-cell sorting method. Specifically, the use of single-cell sorting can achieve 99% purity of the immunophenotyped cells from a heterogeneous population when combined with a multiparametric flow cytometry-based approach.

Education

JoVE Core - Anatomy and Physiology

Teeth

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2024

The formation of teeth, also known as odontogenesis, is a complex process that begins in utero, around the sixth week of embryonic development. There are three stages to this process: the bud stage, the cap stage, and the bell stage. In the bud stage, the tooth germ (an aggregation of cells) starts to form in the developing jawbone. During the cap stage, the tooth germ differentiates into enamel organ, dental papilla, and dental sac, which will later develop into the tooth's enamel, dentin and...

Research

JoVE Journal - Developmental Biology
Free Sample

Isolation of Mouse Endometrial Epithelial and Stromal Cells for In Vitro Decidualization

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Cited by 86 •

2017

This study presents a standardized and validated method for the isolation and culture of primary mouse endometrial stromal and epithelial cells, which can be used in a coculture system to study in vitro decidualization.

Human Placental and Decidual Organ Cultures to Study Infections at the Maternal-fetal Interface

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Cited by 7 •

2016

A simple method to establish primary human placental (villous) and decidual organ cultures is described. Villous and decidual organ cultures are invaluable tools for studying pathogenesis at the human maternal-fetal interface. Infection with the facultative intracellular bacterium Listeria monocytogenes is demonstrated.

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