Dental Plaque Isolation
Dental Plaque Isolation - Related Videos
04:07
Isolation of Epithelial Cells from Human Dental Follicle
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Cited by 1 •
2021
The dental follicle contains an epithelial population and mesenchymal cells. The epithelial population was selected from the heterogeneous dental follicle cell population by providing a distinct culture medium. Epithelial cells survived and formed colonies in a serum-free medium.
09:34
Semi-Automated Planimetric Quantification of Dental Plaque Using an Intraoral Fluorescence Camera
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Cited by 6 •
2023
This study presents a semi-automated digital image analysis procedure for the planimetric quantification of disclosed dental plaque based on images acquired with an intraoral fluorescence camera. The method allows for the rapid and reliable quantification of dental plaque in the research environment.
08:14
Isolation and Culture of Dental Epithelial Stem Cells from the Adult Mouse Incisor
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Cited by 24 •
2014
The continuously growing mouse incisor provides a model for studying renewal of dental tissues from dental epithelial stem cells (DESCs). A robust system for consistently and reliably obtaining these cells from the incisor and expanding them in vitro is reported here.
13:00
Plaque Assay: A Method to Determine Viral Titer as Plaque Forming Units (PFU)
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2023
Source: Tilde Andersson1, Rolf Lood1 1 Department of Clinical Sciences Lund, Division of Infection Medicine, Biomedical Center, Lund University, 221 00 Lund, Sweden Viruses that infect prokaryotic organisms, called bacteriophages or simply phages, were identified in the early 20th century by Twort (1) and d'Hérelle (2) independently. Phages have since been widely recognized for their therapeutic value (3) and their influence on human (4), as well as global, ecosystems (5). Current concerns have...
07:57
Accuracy in Dental Medicine, A New Way to Measure Trueness and Precision
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Cited by 65 •
2014
Accuracy is a major demand in dental medicine. To verify accuracy, reference scanners are needed. This article presents a new reference scanner with an adjusted scanning method to acquire a broad variety of dental morphologies with high trueness and precision.
FAQs
Representative results depend on controlling where and how plaque is collected. Sampling defined oral sites allows investigators to compare biofilms from comparable locations, while standardized handling reduces changes introduced during collection and transfer. Preserving the sample’s microbial composition is important because variation in sampling can be mistaken for a biological difference between sites or subjects.
The downstream analysis determines whether the collected material is placed in an appropriate buffer or a growth medium. Culture-based examination requires conditions that support examination of recoverable microbes, whereas molecular examination uses the preserved sample to characterize microbial communities. Matching handling to the planned analysis helps maintain relevant information and makes results easier to interpret.
Sterile instruments help keep the collection controlled while plaque is removed from a defined oral location. This matters because the sample is intended to represent the microbial biofilm at that site, not an uncontrolled mixture introduced during handling. Careful instrument use therefore supports comparisons among samples and improves confidence in culture-based or molecular findings.
A practical workflow starts by selecting defined oral sites, then uses sterile instruments to dislodge plaque and transfer it into a suitable buffer or growth medium. The material must be handled in a way that preserves its microbial composition before analysis. These linked choices connect the sampled location with the eventual biological measurement.
Comparing healthy and diseased sites allows researchers to ask whether their microbial communities differ in ways associated with oral condition. Such comparisons can be paired with culture-based or molecular examination to characterize the recovered biofilm. In biology, this design helps investigate relationships between plaque-associated microbes and conditions such as caries or periodontal disease.
Beyond describing which microbes are present, isolated plaque samples can support studies of biofilm formation, antimicrobial responses, and host-microbe interactions. These applications place the oral biofilm in a broader biological context: researchers can relate community characteristics to disease-associated sites or to antimicrobial responses observed in the study. Standardized sampling strengthens the resulting comparisons.