Dewar Tank Retrieval
Dewar Tank Retrieval - Related Videos
05:47
Do-It-Yourself Device for Recovery of Cryopreserved Samples Accidentally Dropped into Cryogenic Storage Tanks
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Cited by 5 •
2012
Here we present a low cost, durable cryotolerant device for sample retrieval from Dewar tanks filled with liquid nitrogen. The ease of construction and modular design of the device makes the process of sample retrieval from cryogenic tanks safe and easy.
08:42
Retrieval of Mouse Oocytes
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Cited by 13 •
2007
This protocol illustrates the technique for extracting oocytes or early-stage fertilized embryos from the oviduct of mice. The ability to identify the infindibulum and insert a blunt end needle into it is essential to correctly performing the procedure.
06:06
A Simple Dewar/Cryostat for Thermally Equilibrating Samples at Known Temperatures for Accurate Cryogenic Luminescence Measurements
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2016
A simple liquid nitrogen Dewar/cryostat apparatus comprised of a small fused silica optical Dewar, a thermocouple, and a charge-coupled device (CCD) spectrograph are described. The experiments for which this Dewar/cryostat is designed require fast sample loading, freezing, and alignment, accurate and stable sample temperatures, and small size/portability.
01:12
Retrieval
0 Views •
2025
Retrieval is the process of getting information out of memory storage and back into conscious awareness. This ability is essential for daily tasks like brushing hair and teeth, driving to work, and performing job duties. Retrieval occurs in three ways: recall, recognition, and relearning. Recall involves accessing information without cues, such as during an essay test, where individuals must retrieve facts and concepts from memory unaided. Another example is remembering the name of a colleague...
02:32
Novel Tank Diving Assay: A Method to Study Innate Stress Responses in Zebrafish
0 Views •
2023
The article describes a behavioral method to assess stress in zebrafish. In response to stress, zebrafish tends to swim at bottom of a tank until stress is removed. The sample protocol describes stepwise method for performing the assay.
FAQs
Temperature control is central because cryogenic specimens can lose quality when handling allows them to warm. Maintaining the stored temperature helps protect cell viability, tissue integrity, and the quality of genetic or microbial stocks. This is especially important when materials will undergo downstream analysis, since inconsistent warming can undermine comparability and reduce confidence in experimental results.
Traceability connects the retrieved specimen to its recorded identity, reducing the risk of selecting the wrong material or losing track of its origin. Maintaining this connection supports biosafety, organized biobank operations, and reproducible research. It also allows downstream findings to be interpreted in relation to the correct biological sample.
The vacuum-insulated vessel and its liquid-nitrogen environment provide the cold-storage conditions needed to preserve biological materials. During access, personnel must work in a way that limits warming because the protective storage environment is temporarily being opened or disturbed. Controlling this transition helps maintain sample quality until the material reaches its next research use.
Cryogenic tools and protective equipment address two linked needs: controlled handling of cold-stored material and reduced exposure hazards for personnel. Using appropriate equipment helps staff access canisters or racks while preserving sample identification and limiting unnecessary warming. This supports safer, more consistent retrieval in biological facilities.
A controlled retrieval begins with locating the intended inventory, followed by accessing the appropriate canister or rack. Personnel then remove the biological material with suitable cryogenic tools and protective equipment while maintaining its identification and temperature control. This sequence supports efficient sample management and helps preserve the material for downstream biological work.
Efficient access limits the opportunity for warming, but verification must occur at the same time. Personnel need to locate the correct inventory entry, confirm sample identification, and access the relevant canister or rack using suitable cryogenic tools. Balancing these actions helps preserve temperature control without sacrificing traceability or selecting an incorrect biological material.
Careful retrieval supports biobanks, cell culture studies, and clinical or developmental investigations by preserving the quality of stored biological materials. It helps maintain cell viability, tissue integrity, and genetic or microbial stock quality for downstream analysis. Standardized handling also improves biosafety, sample management, and reproducibility across different research activities.