Retrieval of Mouse Oocytes

Amanda R. Duselis; Paul B. Vrana

doi:10.3791/185

JoVE Journal
Biology

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JoVE Journal Biology

Retrieval of Mouse Oocytes

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08:42 min

April 28, 2007

DOI: 10.3791/185-v

Amanda R. Duselis¹, Paul B. Vrana¹

¹Dept. of Biological Chemistry,University of California, Irvine (UCI)

This protocol illustrates the technique for extracting oocytes or early-stage fertilized embryos from the oviduct of mice. The ability to identify the infindibulum and insert a blunt end needle into it is essential to correctly performing the procedure.

My name is Amanda dli. I'm from the laboratory of Dr.Paul Verana at the University of California Irvine. Today I'm gonna be showing you the procedure for oocyte retrieval.

This entails isolating the videx from the female. Once the ovex are isolated, we'll be injecting FHM media through the videx, and then cytes should be exiting from the uterus. Once this is done, we'll isolate the cytes in the media and use this for future experiments.

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