Disc Isolation
Disc Isolation - Related Videos
14:33
Generation, Purification, and Characterization of Cell-invasive DISC1 Protein Species
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Cited by 13 •
2012
The generation, purification and cell invasion of intracellular, cytoplasmic full length DISC1 protein aggresomes from cell cultures and of a labeled, multimeric recombinant DISC1 protein fragment in E. coli are described. Cell invasiveness is shown for recipient cells in cell culture and for neurons in vivo after stereotactical brain inoculation.
08:09
Vasodilation of Isolated Vessels and the Isolation of the Extracellular Matrix of Tight-skin Mice
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Cited by 1 •
2017
We describe the isolation of cardiac extracellular matrix from C57Bl/6J control mice, tight-skin mice, and tight-skin mice treated with the IRF5 inhibitory peptide. We also describe the vasodilation studies on the isolated vessels from C57Bl/6J, tight-skin mice and tight-skin mice treated with the IRF5 inhibitory peptide.
07:12
Neutrophil Isolation Protocol
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Cited by 173 •
2008
Neutrophils are among the first cells to arrive on the site of inflammatory immune response, and their functions and mechanisms have been studied extensively in vitro. We demonstrate a standard density gradient separation method to isolate human neutrophils from whole blood using commercially available separation media.
13:24
An Optimized Enrichment Technique for the Isolation of Arthrobacter Bacteriophage Species from Soil Sample Isolates
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Cited by 31 •
2015
We present an enrichment protocol for the isolation of bacteriophages infecting bacteria in the Arthrobacter genus. This enrichment protocol produces fast and reproducible results for the isolation and amplification of Arthrobacter phages from soil isolates.
05:09
Mitochondrial Isolation: A Technique to Isolate Mitochondria from Human Ovarian Cancer Tissue Sample
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2023
This video describes the procedure for isolating mitochondria from human ovarian cancer tissues using differential-speed centrifugation in combination with density gradient centrifugation. The isolated mitochondria can be used for proteomic analysis of human ovarian cancer mitochondrial proteome.
FAQs
Preserving the annulus fibrosus and nucleus pulposus is central because these components represent the disc’s main internal organization. Their retention allows investigators to examine the tissue as a defined preparation rather than interpreting results from incomplete or disrupted material. This structural continuity supports studies of disc development, degeneration, biomechanics, and cellular responses under controlled laboratory conditions.
Mechanical or structural damage introduced during dissection could alter the preparation before analysis. Careful isolation therefore helps distinguish biological features of the disc from changes caused by removal. This distinction is especially relevant when researchers examine tissue degeneration, biomechanics, or cellular responses, where the condition of the isolated specimen can influence how experimental findings are interpreted.
Using a defined preparation helps researchers study the disc within a controlled laboratory setting after separating it from surrounding structures. This organization is valuable when structural, molecular, cellular, and mechanical questions overlap. It allows investigators to relate findings from histology or molecular assays to the condition and behavior of the isolated disc rather than to a mixed tissue sample.
The dissection focuses on separating the disc from adjacent vertebral and connective tissues while retaining the annulus fibrosus and nucleus pulposus. This selective approach creates a specimen that can be studied independently and reduces the chance that neighboring structures or dissection-related damage will confound observations. The result is a more controlled basis for downstream biological and mechanical analyses.
After isolation, researchers can use the specimen for organ culture, histological analysis, or molecular assays. These approaches examine different levels of biology: culture supports controlled study of tissue behavior, histology reveals structural features, and molecular assays assess biological changes. Together, they allow disc development, degeneration, and cellular responses to be investigated within the same defined experimental preparation.
Disc isolation is useful when researchers need to connect tissue structure with function or disease-related change. Isolated specimens support investigations of degeneration and biomechanics, while also enabling study of disc development and cellular responses. The preparation can further be used when evaluating potential therapeutic strategies, because experiments can be conducted on a defined tissue system under controlled laboratory conditions.