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JoVE Journal
Biology

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Generation, Purification, and Characterization of Cell-invasive DISC1 Protein Species
 

Generation, Purification, and Characterization of Cell-invasive DISC1 Protein Species

Article DOI: 10.3791/4132
August 30th, 2012

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Summary August 30th, 2012

The generation, purification and cell invasion of intracellular, cytoplasmic full length DISC1 protein aggresomes from cell cultures and of a labeled, multimeric recombinant DISC1 protein fragment in E. coli are described. Cell invasiveness is shown for recipient cells in cell culture and for neurons in vivo after stereotactical brain inoculation.

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