Dissecting Microscope Observation
Dissecting Microscope Observation - Related Videos
10:41
Continuous High-resolution Microscopic Observation of Replicative Aging in Budding Yeast
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Cited by 10 •
2013
We describe here the operation of a microfluidic device that allows continuous and high-resolution microscopic imaging of single budding yeast cells during their complete replicative and/or chronological lifespan.
04:58
Heart Dissection: A Method to Observe Zebrafish Cardiac Development
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2023
This video describes a technique of isolating the heart from a zebrafish at multiple developmental stages to study cardiac development, maturation, and ageing.
03:12
Drosophila Larva Imaginal Disc Dissection: A Method to Observe Developing Epithelia
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2023
When staining Drosophila imaginal discs, often a rough dissection is performed where the discs are exposed but remain attached to the body. Once stained, the discs of interest are isolated and mounted. The example protocol demonstrates a procedure for wing imaginal disc dissection and mounting.
06:31
Microscopic Observation of Lymphocyte Dynamics in Rat Peyer's Patches
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Cited by 3 •
2020
Here, we describe a precise method for collecting thoracic duct lymphocytes and observing the migration of gut-tropic lymphocytes in rat Peyer’s patches for 3 hours using time-lapse photography. This technique can clarify how the dynamics of lymphocytes are affected under inflammatory conditions.
06:33
Multiphoton Microscopic Observation of Vessels in Mouse Liver Tissue
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2021
In this experiment, a mouse is injected in its tail vein with Rhodamine B isothiocyanate–dextran that can stain blood vessels. After the liver is exposed and fixed, a specific part of the liver can be selected to observe the deep tissue in the living body using multiphoton microscopy.
FAQs
Each eye receives a view through its own optical path, and the brain combines these slightly different views into a three-dimensional image. This depth perception helps the observer judge the relative position of external structures rather than seeing only a flat projection. It is especially useful when examining complex surfaces or guiding precise manipulation of a specimen.
Reflected illumination directs light onto the specimen’s surface, making external features easier to examine, while transmitted illumination passes light through the specimen and can reveal features that depend on light passing across or through it. Choosing between these illumination approaches helps match viewing conditions to the specimen and the structures being investigated.
Low magnification preserves a broad view of a relatively large specimen, allowing structures and their spatial relationships to remain visible together. The instrument’s working distance provides room between the objective and specimen, so the observer can handle or manipulate the sample while viewing it. These features support examination without sectioning and facilitate practical biological work.
The method can reveal external morphology, movement, spatial relationships, and developmental features in whole specimens. Its use extends across plants, insects, embryos, tissues, and small organisms, depending on the sample and the observation goal. Because the specimen remains intact, researchers and students can examine how visible parts are arranged in relation to one another.
A basic workflow begins by positioning the whole specimen for viewing, selecting reflected or transmitted illumination, and using low magnification to locate the relevant region. The observer then examines external structures and their relationships while maintaining sufficient working distance for handling. This sequence supports both general inspection and more targeted manipulation, such as sorting or microdissection.
It is useful when samples must be inspected as intact objects before being separated, manipulated, or dissected. Sorting can rely on visible external characteristics, whereas microdissection and other precise manipulations benefit from the three-dimensional view and available working distance. In biology teaching and research, the same setup can connect structural observation with hands-on examination of whole specimens.