Duodenal Aspirate
Duodenal Aspirate - Related Videos
06:43
Fluid Aspiration from Mouse Glomeruli: A Method to Aspirate Glomerular Fluid from Mouse Using Two-Photon Mediated Micropuncture
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2023
This video showcases fluid aspiration from mouse glomeruli using the two-photon mediated micropuncture technique. The technique provides access to the living mouse’s glomeruli and thus helps gain insights into renal physiology.
07:10
Femoral Bone Marrow Aspiration in Live Mice
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Cited by 18 •
2014
This protocol describes a procedure for serial sampling of femoral bone marrow (BM) without requiring the sacrifice of mice. This procedure facilitates longitudinal studies of the BM composition of mice over time and provides serial access to cells within the BM for ex vivo and transplantation studies.
06:41
A Technique for Percutaneous Epididymal Spermatozoa Aspiration and Processing
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2026
Source:Li Xiaoming1, Liu Xiao1, Li Haiyang1, Cai Xiaohua1, Ou Jianping11Center for Reproductive Medicine, The Third Affiliated Hospital of Sun Yat-sen University.This video demonstrates the percutaneous aspiration of epididymal spermatozoa from a patient with azoospermia, a condition characterized by the absence of spermatozoa in the semen. The procedure involves disinfecting the scrotal area, aspirating epididymal fluid using a syringe with a specialized culture solution and processing the...
10:41
Robotic D3 Partial Duodenal Resection with Primary Side-to-Side Anastomosis
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2023
This protocol presents a case of a robotic partial duodenal resection with primary side-to-side duodeno-jejunal reconstruction in a patient with a 5 cm duodenal stenosis. This is done at the third duodenal segment (D3) after an endoscopic mucosal resection (EMR) for a duodenal polyp.
02:43
Fly Aspirator Tube: A Simple Tool to Transfer Live Drosophila
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2023
This video describes one method to build a fly aspirator, which can be used to transfer insects between enclosures without anesthesia. The aspirator in the example protocol was prepared for an experiment to quantify individual grooming...
FAQs
Accurate positioning in the duodenum and gentle suction are central to preserving the specimen’s biological relevance. An endoscope or nasoduodenal tube reaches the intended intestinal region, while careful aspiration reduces carryover from the mouth and stomach. This approach helps researchers examine material that more closely represents the small-intestinal environment rather than contamination from upstream sites.
A collected specimen can support several complementary analyses. Microscopy allows direct examination of cells, suspended material, or possible parasites; culture can investigate microorganisms that grow under laboratory conditions; and molecular analysis can detect biological signals in the sample. Using these approaches, investigators can study intestinal microorganisms and parasitic infections from the same type of biological material.
The duodenum contains information about processes occurring early in the small intestine, including the presence of bile, digestive secretions, and material undergoing nutrient processing. Sampling this location provides direct evidence about the local intestinal environment. In biology research, that evidence can help connect gastrointestinal physiology with host-microbe interactions and mechanisms associated with disease.
Collection begins by positioning an endoscope or nasoduodenal tube in the duodenum. Once the instrument reaches the intended site, gentle suction retrieves intestinal fluid and suspended material. The specimen can then be prepared for microscopic examination, culture, or molecular analysis. Careful placement and aspiration are important because the goal is to study duodenal contents while minimizing upstream contamination.
Researchers may select this specimen when they need biological material from the small-intestinal environment to investigate intestinal microorganisms or parasitic infections. Microscopy, culture, and molecular analysis provide different ways to examine the collected contents. The resulting information can support studies of infection-related biology and help relate organisms detected in the sample to conditions within the duodenum.
In addition to investigating microorganisms and parasites, duodenal aspiration supports research on gastrointestinal physiology, host-microbe interactions, and disease mechanisms. The sample may also be examined for bile, digestive secretions, and material related to nutrient processing. Because it comes directly from the duodenum, the specimen links these biological features to processes occurring in the first segment of the small intestine.