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Femoral Bone Marrow Aspiration in Live Mice
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Femoral Bone Marrow Aspiration in Live Mice

Femoral Bone Marrow Aspiration in Live Mice

DOI:
10.3791/51660-v

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07:10 min

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July 05, 2014

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, ,
1Human Oncology and Pathogenesis Program, Department of Medicine,Memorial Sloan-Kettering Cancer Center

Chapters

  • 00:05Title
  • 01:12Aspiration and Assessment of Bone Marrow (BM) Cells from the Femur
  • 04:57Results: Representative BM Aspirate Mononuclear Cell Analyses
  • 06:38Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes a procedure for serial sampling of femoral bone marrow (BM) without requiring the sacrifice of mice. This procedure facilitates longitudinal studies of the BM composition of mice over time and provides serial access to cells within the BM for ex vivo and transplantation studies.

Tags

Bone Marrow AspirationFemoral Bone MarrowHematopoietic Stem CellsHematopoietic Progenitor CellsStem Cell CharacterizationSerial SamplingLive Mouse ModelXenograft Studies

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