Elisa Conversion
Elisa Conversion - Related Videos
10:06
The ELISA Method
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2023
An enzyme-linked immunosorbent assay (ELISA) is typically performed to detect the presence and/or amount of a target protein of interest within an experimental sample. Detection of the target protein is made possible by antibodies, which make the ELISA an immunoassay. Through a series of incubation and washing steps, these antibodies, which are frequently linked, or conjugated, to an enzyme, will detect protein coating the bottom of a well on a microtiter plate. When exposed to a substrate,...
14:48
Conversion of a Capture ELISA to a Luminex xMAP Assay using a Multiplex Antibody Screening Method
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Cited by 53 •
2012
An ELISA can be easily converted to a Luminex xMAP assay and, through the benefits of multiplexing, several antibodies can be screened simultaneously to identify an optimum antibody pair, resulting in increased sensitivity and dynamic range, while reducing assay cost.
14:22
ELISA Assays: Indirect, Sandwich, and Competitive
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2023
Source: Whitney Swanson1,2, Frances V. Sjaastad2,3, and Thomas S. Griffith1,2,3,4 1 Department of Urology, University of Minnesota, Minneapolis, MN 55455 2 Center for Immunology, University of Minnesota, Minneapolis, MN 55455 3 Microbiology, Immunology, and Cancer Biology Graduate Program, University of Minnesota, Minneapolis, MN 55455 4 Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455 Enzyme-linked immunosorbent assay (ELISA) is frequently used to measure the presence...
02:08
Gene Conversion
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2020
Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
12:38
Titration ELISA as a Method to Determine the Dissociation Constant of Receptor Ligand Interaction
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Cited by 29 •
2018
A detailed protocol to perform a titration ELISA is described. Moreover, a novel algorithm is presented to evaluate titration ELISAs and to obtain a dissociation constant of binding of a soluble ligand to a microtiter plate-immobilized receptor.
FAQs
A standard curve links measured optical density to known analyte concentrations, providing the reference needed to interpret an unknown sample. After the assay generates an enzyme-dependent color signal, the sample absorbance is compared with this curve to estimate concentration. This approach converts an instrument reading into a biologically meaningful quantitative result rather than treating absorbance as concentration itself.
Controls help indicate whether the measured signal can be interpreted reliably, while the assay range identifies concentrations for which the standard curve is appropriate. If a sample falls outside that range, its estimate may not be dependable. Checking both features helps distinguish a meaningful analyte measurement from a value produced under unsuitable assay conditions.
A measured concentration from a diluted sample represents the diluted preparation, not necessarily the original sample. ELISA conversion therefore requires applying the relevant dilution factor after obtaining the estimate from the standard curve. This correction allows the reported value to reflect the starting biological sample and is especially important when dilution was used to place analyte levels within the assay range.
Because conversion depends on comparing optical density values, inconsistent signal measurement can alter the relationship between sample readings and the standard curve. Consistent measurement makes values more comparable across standards and unknown samples, strengthening the concentration estimate. In biological analysis, this improves confidence that observed differences reflect analyte levels rather than variation in signal reading.
To perform the conversion, measure the optical density for the standards and unknown sample, compare the unknown reading with the standard curve, and obtain its corresponding concentration. Then account for any sample dilution factor. Finally, confirm that the value falls within the assay range and that controls and signal measurements support interpretation.
ELISA conversion supports quantitative analysis across several biological contexts, including measurements of proteins, hormones, antibodies, pathogens, and other biological molecules. The resulting concentration estimate can be used in research, diagnostics, and biotechnology, where knowing the amount of a target is more informative than recording a color signal or absorbance value alone.