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Conversion of a Capture ELISA to a Luminex xMAP Assay using a Multiplex Antibody Screening Method
JoVE Journal
Biology
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JoVE Journal Biology
Conversion of a Capture ELISA to a Luminex xMAP Assay using a Multiplex Antibody Screening Method

Conversion of a Capture ELISA to a Luminex xMAP Assay using a Multiplex Antibody Screening Method

DOI:
10.3791/4084-v

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14:48 min

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July 06, 2012

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, , ,
1Chemistry Research and Development,Luminex Corporation, 2Global Marketing,Luminex Corporation

Chapters

  • 00:05Title
  • 01:29Coupling Antibodies to MagPlex Microspheres, using the xMAP Antibody Coupling Kit
  • 04:58Antibody Screening
  • 07:20xMAP Functional Assay
  • 09:07ELISA Assay
  • 09:44Results: Comparison of an ELISA Assay for TNF-alpha Cytokine to the Converted Assay using the xMAP Platform
  • 14:28Conclusion

Summary

Automatic Translation

Automatic Translation

An ELISA can be easily converted to a Luminex xMAP assay and, through the benefits of multiplexing, several antibodies can be screened simultaneously to identify an optimum antibody pair, resulting in increased sensitivity and dynamic range, while reducing assay cost.

Tags

ELISALuminex XMAP AssayAntibody ScreeningMultiplexCapture AntibodyAnalytesBiological SamplesLife Science ResearchClinical DiagnosticsLimitations96-well MicroplateSample VolumeAntigenNon-specific BindingBackgroundEnzyme-mediated AmplificationSensitivityTechnologyHigher ThroughputFlexibilityReduced CostWorkflowLuminex XMAP TechnologyMicrosphere Array PlatformMonoplex AssayProtein ApplicationsNucleic Acid Applications

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