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JoVE Journal
Biology

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Conversion of a Capture ELISA to a Luminex xMAP Assay using a Multiplex Antibody Screening Method
 

Conversion of a Capture ELISA to a Luminex xMAP Assay using a Multiplex Antibody Screening Method

Article DOI: 10.3791/4084 14:48 min
July 6th, 2012

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Summary July 6th, 2012

An ELISA can be easily converted to a Luminex xMAP assay and, through the benefits of multiplexing, several antibodies can be screened simultaneously to identify an optimum antibody pair, resulting in increased sensitivity and dynamic range, while reducing assay cost.

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