Epi300t1 Resistant Cells
Epi300t1 Resistant Cells - Related Videos
10:54
A Cell Culture Model of Resistance Arteries
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Cited by 5 •
2017
A cell culture model of resistance arteries is described, allowing for the dissection of signaling pathways in endothelium, smooth muscle, or between endothelium and smooth muscle (the myoendothelial junction). The selective application of agonists or protein isolation, electron microscopy, or immunofluorescence can be utilized using this cell culture model.
15:40
Forward Genetic Approach to Uncover Stress Resistance Genes in Mice — A High-throughput Screen in ES Cells
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2015
Stress resistance is one of the hallmarks for longevity and is known to be genetically governed. Here, we developed an unbiased high-throughput method to screen for mutations that confer stress resistance in ES cells with which to develop mouse models for longevity studies.
04:17
Dose Escalation: A Method for Developing Drug Resistance in Cancer Cells
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2023
The video describes the development of drug resistance in cancer cells by exposing cells to escalating doses of a drug after calculating IC50 values of the drug previously with MTT assay. The sample protocol describes the step-by-step process of developing drug-resistant cells.
05:01
Multiplexed Immunofluorescence Imaging to Analyze Immunotherapy-Resistant T Cell Subpopulations
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2025
This video demonstrates an in-situ fluorescence multispectral imaging technique to analyze different subpopulations of infiltrating T cells in tumor tissue. A renal cell carcinoma tissue cryosection is treated with antibodies to label CD8+ T cells that express specific inhibitory receptors and exhibit an immunotherapy-resistant phenotype. The labeled section is visualized using a multispectral microscope to identify the distinct CD8+ T cell subpopulations.
01:22
Resistivity
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2023
When a voltage is applied to a conductor, an electrical field is generated, and charges in the conductor feel the force due to the electrical field. The current density that results depends on the electrical field and the properties of the material. In some materials, including metals at a given temperature, the current density is approximately proportional to the electrical field. In these cases, the current density can be modeled as: where σ is the electrical conductivity and is represented...
FAQs
T1 resistance limits bacteriophage-mediated infection and cell lysis, allowing cultures carrying recombinant DNA to remain intact during propagation. This protection is especially relevant when a culture contains valuable or complex plasmid assemblies, because preserving viable cells helps maintain the DNA source for later plasmid recovery and downstream molecular genetics experiments.
The inducible copy mechanism separates routine maintenance from later plasmid amplification. After culture conditions trigger replication control, the number of vector molecules increases within the cells, which can improve plasmid yield without changing the recombinant insert itself. This feature is useful when a construct is maintained at low copy but more DNA is needed for analysis.
Large-insert DNA constructs and low-copy plasmids can be difficult to recover reliably in standard cloning strains. Epi300T1 Resistant Cells are suited to maintaining these complex molecules while supporting later amplification, helping preserve genomic libraries, bacterial artificial chromosomes, and other assemblies whose integrity is important for sequencing or functional studies.
They are most relevant when the target plasmid contains a large insert, belongs to a genomic library, or is designed for low-copy maintenance. The overview indicates that such molecules may be difficult to recover in standard strains, so this system offers a more specialized option for preserving and amplifying complex recombinant DNA.
The workflow involves maintaining the recombinant plasmid in the engineered Escherichia coli, culturing the cells under conditions that preserve the construct, and then triggering the plasmid-copy mechanism when increased vector abundance is needed. The resulting culture can provide recovered plasmid DNA for sequencing, functional studies, or other downstream molecular genetics applications.
This system supports work with genomic libraries, bacterial artificial chromosomes, and other complex DNA assemblies. Increased plasmid yield can provide material for sequencing and functional studies, while stable propagation helps preserve the constructs before those experiments. Its value therefore extends from DNA library maintenance to analysis of recombinant sequences and their biological roles.