Epoxy Sealing Technique

Epoxy sealing technique is a laboratory method that uses a curing epoxy resin to create a durable, leak-resistant barrier between surfaces, helping preserve sample integrity and maintain controlled experimental conditions. The resin flows into gaps, adheres to surrounding materials, and hardens through a chemical curing reaction that forms a cross-linked polymer network. In biology, this approach can seal microscope chambers, specimen holders, culture devices, and other experimental assemblies, reducing fluid loss, contamination, and exposure to the environment. Reliable sealing supports microscopy, sample preservation, and reproducible studies involving fluids, tissues, cells, or biological materials.

Epoxy Sealing Technique - Related Videos

Research

JoVE Journal - Engineering

Optimized Sealing Process and Real-Time Monitoring of Glass-to-Metal Seal Structures

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Cited by 7 •

2019

Key procedures to optimize the sealing process and achieve real-time monitoring of the metal-to-glass seal (MTGS) structure are described in detail. The embedded fiber Bragg grating (FBG) sensor is designed to achieve online monitoring of temperature and high-level residual stress in the MTGS with simultaneous environmental pressure monitoring.

Research

JoVE Journal - Medicine
Free Sample

The C-seal: A Biofragmentable Drain Protecting the Stapled Colorectal Anastomosis from Leakage

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Cited by 8 •

2010

The C-seal is a biofragmentable drain protecting the stapled colorectal anastomosis. In this video, details of the manufacturing, deployment and use are shown.

Accessible Silicone Chip-to-Membrane Sealing Procedure for Flexible, Reliable Bonding

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2026

A novel sealing protocol to produce water-tight silicone-based seals for membranes of Polydimethylsiloxane (PDMS) chip devices. Intended for labs setting-up chip models and small-scale chip platform development. We demonstrate the protocol using plastic and native tissue membranes. This procedure only requires PDMS, toluene, mold, membrane, and a vacuum chamber.

Achieving Moderate Pressures in Sealed Vessels Using Dry Ice As a Solid CO2 Source

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Cited by 11 •

2018

Here we present a protocol for performing reactions in simple reaction vessels under low-to-moderate pressures of CO2. The reactions can be performed in a variety of vessels simply by administering the carbon dioxide in the form of dry ice, without the need for costly or elaborate equipment or set-ups.

Vacuum-Sealed Hot Water Bath Immersion for the Preparation of Anatomical and Surgical Cadaveric Bone Models

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Cited by 1 •

2022

The present protocol describes the maceration and cleaning of cadaveric bone with a vacuum-sealed, hot water bath immersion technique. This is a low-cost, safe, and effective method to produce anatomical specimens for surgical planning and medical education as an alternative to three-dimensional (3D) printed models.

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