Eye Phenotype Analysis

Eye phenotype analysis is the systematic characterization of observable eye traits, such as structure, pigmentation, size, and visual function, to understand variation in biological systems. It combines standardized observation or imaging with quantitative measurements, allowing researchers to compare phenotypes and relate them to genetic factors, development, disease, or environmental conditions. In biology, this approach supports studies of inheritance, embryonic development, model organisms, and ocular disorders by revealing how changes in cells, tissues, or genes affect eye form and performance. Consistent phenotyping also enables researchers to evaluate experimental outcomes and identify relationships between genotype and phenotype.

Eye Phenotype Analysis - Related Videos

Research

JoVE Journal - Medicine

Mouse Eye Enucleation for Remote High-throughput Phenotyping

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Cited by 19 •

2011

The dissection technique illustrates enucleation of the mouse eye for tissue fixation to perform phenotyping in high-throughput screens.

Research

JoVE Journal - Biology
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Preparation of Adult Drosophila Eyes for Thin Sectioning and Microscopic Analysis

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Cited by 21 •

2011

A standard approach to prepare adult Drosophila eyes for semi-thin sectioning and light microscopic analysis is presented here. The protocol can be used for gross morphological analysis of eye defects, or with the indicated adjustments can be used to determine genetic requirements of genes in specific cell types of the eye (e.g. clonal analysis of photoreceptors) or for electron microscopic analysis.

Lens Transplantation in Zebrafish and its Application in the Analysis of Eye Mutants

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Cited by 4 •

2009

Lens development involves interactions with other tissues. Several zebrafish eye mutants are characterized by an abnormally small lens size. Here we demonstrate a lens transplantation experiment to determine whether this phenotype is due to intrinsic causes or defective interactions with tissues that surround the lens.

Automated Analysis of Intracellular Phenotypes of Salmonella Using ImageJ

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Cited by 3 •

2022

Salmonella invades and replicates inside intestinal epithelial cells both in Salmonella-specific vacuoles and free in the cytosol (hyper-replication). A high-throughput fluorescence microscopy-based protocol is described here to quantify the intracellular phenotypes of Salmonella by two complementary image analyses through ImageJ, reaching single-cell resolution and scoring.

A Non-invasive Way to Isolate and Phenotype Cells from the Conjunctiva

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Cited by 5 •

2017

The exposed normal ocular surface consists of cornea and conjunctiva. Epithelial cells, goblet cells and immune cells are present in the conjunctiva. Here, a non-invasive, technique of impression cytology is described using an impression cytology device and flow cytometry to analyze immune cells in the conjunctiva.

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