Fibroblast Proliferation
Fibroblast Proliferation - Related Videos
07:03
Determining Genome-wide Transcript Decay Rates in Proliferating and Quiescent Human Fibroblasts
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Cited by 4 •
2018
We describe a protocol for generating proliferating and quiescent primary human dermal fibroblasts, monitoring transcript decay rates, and identifying differentially decaying genes.
09:43
Isolation, Culture, and Characterization of Prostate Cancer-Associated Fibroblasts
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Cited by 1 •
2025
This protocol provides a comprehensive procedure to isolate, expand, and immortalize fibroblasts from radical prostatectomies. Moreover, it describes assays developed to assess the functional effects of fibroblast-tumor cell crosstalk, taking advantage of both treatment with conditioned medium and co-culturing.
15:43
Experimental Generation of Carcinoma-Associated Fibroblasts (CAFs) from Human Mammary Fibroblasts
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Cited by 25 •
2011
Carcinoma-associated fibroblasts (CAFs) rich in myofibroblasts present within the tumour stroma, play a major role in driving tumour progression. We developed a coimplantation tumour xengraft model for experimentally generating CAFs from human mammary fibroblasts. The protocol describes how to establish CAF myofibroblasts that acquire an ability to promote tumourigenesis.
02:23
Abnormal Proliferation
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2021
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
11:43
Establishing Primary Adult Fibroblast Cultures From Rodents
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Cited by 305 •
2010
This article describes a protocol for isolation and maintenance of primary fibroblast cultures from skin and lung tissue of wild rodents.
FAQs
Platelet-derived growth factor and transforming growth factor beta act as important injury-associated signals in the described response. They can activate fibroblasts and promote progression through the cell cycle, increasing the cellular contribution to repair. At the same time, these signals stimulate extracellular matrix production, including collagen, linking proliferation with remodeling.
Cell-cycle progression increases the number of fibroblasts available to participate in tissue repair, while extracellular matrix production supplies structural components for remodeling. Collagen is a key example of this matrix output. Considering both processes together helps explain why fibroblast activity can support restoration when regulated appropriately, rather than treating cell number as the only outcome.
Fibroblast activity becomes problematic when proliferation and matrix production remain excessive or poorly controlled after repair needs have been addressed. Continued activity can promote excess collagen accumulation and contribute to scar formation or pathological fibrosis. This distinction makes regulation important: the same cellular responses that assist healing can produce disease-associated remodeling when they persist.
Measurements can show how fibroblasts respond to injury-related signals, biomaterials, or candidate therapies. Interpreting changes in proliferation alongside the broader repair response can help researchers assess whether a condition supports controlled tissue remodeling or favors excessive activity. Such measurements therefore provide evidence relevant to wound healing, fibrosis, and regenerative medicine studies.
The process is especially informative in research on connective tissue repair, wound healing, tissue engineering, and fibrosis. It can also help evaluate how fibroblasts respond to biomaterials or candidate therapies. These applications use changes in fibroblast activity to investigate repair mechanisms, identify potentially harmful overactivity, and examine strategies for regulating tissue remodeling.
Researchers can examine whether biomaterials or candidate therapies alter fibroblast proliferation and the associated production of extracellular matrix components such as collagen. A response consistent with controlled repair may support regenerative applications, whereas persistent or excessive activity may raise concerns about scarring or pathological fibrosis. This makes fibroblast behavior a relevant biological readout.