Determining Genome-wide Transcript Decay Rates in Proliferating and Quiescent Human Fibroblasts

Mithun Mitra; Ha Neul Lee; Hilary A. Coller

doi:10.3791/56423

Journal

/

Genetics

/

Determining Genome-wide Transcript Decay Rates in Proliferating and Quiescent Human Fibroblasts

JoVE Journal
Genetics

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JoVE Journal Genetics

Determining Genome-wide Transcript Decay Rates in Proliferating and Quiescent Human Fibroblasts

6,164 Views

•

07:03 min

•

January 02, 2018

DOI:

10.3791/56423-v

DOI:

10.3791/56423-v

•

07:03 min

January 02, 2018

•

6155 Views

Mithun Mitra^1,2, Ha Neul Lee^1,2,3, Hilary A. Coller^1,2,3

¹Department of Molecular, Cell and Developmental Biology,University of California, Los Angeles ²Department of Biological Chemistry,David Geffen School of Medicine ³Molecular Biology Institute Interdepartmental Program,University of California, Los Angeles

Summary

We describe a protocol for generating proliferating and quiescent primary human dermal fibroblasts, monitoring transcript decay rates, and identifying differentially decaying genes.

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Determining Genome-wide Transcript Decay Rates in Proliferating and Quiescent Human Fibroblasts

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