Field Potential Recording
Field Potential Recording - Related Videos
03:00
Recording of Local Field Potential from Both Hemispheres of the Mouse Brain
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2025
This video demonstrates in vivo recording of local field potential (LFP) from bilateral M2 areas of the mouse brain. An anesthetized mouse is secured and prepared for the procedure. To record LFPs, insert microelectrodes into the target coordinates of the bilateral M2 areas, which are responsible for complex motor movements.
05:26
Recording Neuronal Field Excitatory Postsynaptic Potentials in Acute Hippocampal Slices
0 Views •
2025
This video demonstrates the procedure for setting up a brain slice in a submersion chamber to record synaptic activity. The process involves securing the slice on lens paper, maintaining a continuous flow of artificial cerebrospinal fluid (aCSF) to ensure neuronal viability, and accurately positioning the stimulation and recording electrodes relative to a specific brain region of interest. This setup allows for precise monitoring of baseline synaptic activity and the effects of electrical...
02:44
Recording of Local Field Potential in Mouse Hippocampal-Entorhinal Cortex Slices
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2025
The video demonstrates a procedure to record the local field potential (LFP) in mouse hippocampal-entorhinal cortex slices. Electrical stimulation is provided at the CA3 stratum radiatum of the hippocampus, which causes changes in the postsynaptic potential at the CA1 stratum pyramidale. The combined change in the membrane potential of CA1 neurons, termed the local field potential, is recorded.
04:17
Concurrent Electroencephalographic and Local Field Potential Recordings in an Anesthetized Rat
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2025
This video demonstrates the procedure for concurrent recordings of co-localized electroencephalography (EEG) and local field potentials (LFPs) in an anesthetized rat. The process involves preparing the rat and attaching stimulating electrodes to its whisker pads. An EEG spider electrode is placed on the rat's skull for surface EEG measurements. At the same time, a multi-channel microelectrode is inserted into a specific brain region through a burr hole in the skull to record neural activity...
08:31
Concurrent Recording of Co-localized Electroencephalography and Local Field Potential in Rodent
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Cited by 4 •
2017
This protocol describes a simple method for concurrent recording of co-localized electroencephalography (EEG) and multi-laminar local field potential in an anesthetized rat. A burr hole drilled in the skull for the insertion of a microelectrode is shown to produce negligible distortion of the EEG signal.
FAQs
Electrode placement in the extracellular space allows the recording to reflect voltage gradients produced by ionic currents from multiple electrically active cells. When synaptic activity and action potentials occur in coordinated patterns, their effects contribute to a population-level signal. This makes the technique useful for examining tissue-wide function rather than only one cellular event.
Field Potential Recording depends on the relationship between ionic current flow and the surrounding extracellular space. Those currents create voltage gradients that an electrode can detect, while amplification increases the measurable signal and filtering separates distinct signal components. Together, these steps make synaptic and action-potential-related activity easier to analyze in the recorded trace.
The interpretation depends on the electrically active processes contributing to the extracellular voltage. Synaptic activity and action potentials generate ionic currents with different contributions to the recorded signal, and amplification and filtering help reveal those components. Consequently, researchers can relate features of a trace to population synaptic transmission or broader network excitability.
A basic workflow begins by placing an electrode in or near the biological tissue being studied. The electrode detects extracellular voltage changes and gradients generated by ionic currents. The recorded signal is then amplified so it can be measured clearly, while filtering helps reveal distinct components associated with the tissue’s electrical activity.
Field potential recordings can be applied in brain slices and intact neural tissue, allowing researchers to examine electrical function in both organized experimental preparations and functioning tissue. In these settings, the measurements support assessment of synaptic transmission, network excitability, and circuit organization, so the same general approach can address complementary biological questions.
Researchers can use changes in field potential patterns to identify altered tissue function associated with neurological disease, drug exposure, or other experimental manipulation. Because the measurements report population-level electrical activity, they can show changes in synaptic transmission, network excitability, or circuit organization. Comparisons with normal physiology help place those functional changes in context.