Recording of Local Field Potential from Both Hemispheres of the Mouse Brain

Begin with an anesthetized mouse with its head secured in a stereotactic frame.

Shave and sterilize the scalp.

Make an incision and remove the residual tissue to expose the skull.

Clean the skull with a disinfectant and drill holes at the target locations.

Remove the outer layer of the brain.

Insert microelectrodes filled with an ionic solution into the holes at a desired angle.

Lower both microelectrodes into the target coordinates of the brain.

Test the electrodes' resistance and adjust the recording settings to ensure the reliable acquisition of local field potential or LFP data.

LFP reflects voltage changes resulting from the collective activity of many neurons within a small region.

Record LFPs from both hemispheres to compare the neural activity between them.

Prior to surgery, confirm the depth of anesthesia of the mouse by performing a tail or toe pinch with forceps. Next, position the mouse in a stereotaxic apparatus, and fix its head. Apply ointment on both eyes to keep them moist.

Then, shave the head and sterilize the area. Make a small incision of 12 to 15 millimeters in the middle of the shaved area. Using forceps, gently pull the scalp away from the midline. Afterward