Fluorescence Detection
Fluorescence Detection - Related Videos
03:44
Detection of Inflammasome Activation via Fluorescence Microscopy
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2025
This video demonstrates a method to detect NLRP3 inflammasome activation in macrophages using fluorescent reporter probes. The cells are treated with potassium ionophores and glycine to induce inflammasome assembly and caspase-1 activation. Fluorescent reporter probes visualize activated caspase-1, and cells are stained with a fluorescent dye for nuclei visualization.
14:16
Fluorescence detection methods for microfluidic droplet platforms
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Cited by 20 •
2011
Droplet-based microfluidic platforms are promising candidates for high throughput experimentation since they are able to generate picoliter, self-compartmentalized vessels inexpensively at kHz rates. Through integration with fast, sensitive and high resolution fluorescence spectroscopic methods, the large amounts of information generated within these systems can be efficiently extracted, harnessed and utilized.
03:08
Detecting Bacterial Contamination Using Magneto-fluorescent Nanosensors
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2025
This video demonstrates a technique to detect bacterial contaminants in food and water samples using nanosensors. The target pathogenic bacteria are detected through a combination of magnetic relaxation and fluorescence emission modalities.
09:10
Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce
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Cited by 17 •
2010
This protocol describes a simple adhesive-tape-based approach for sampling of tomato and other fresh produce surfaces, followed by rapid whole cell detection of Salmonella using fluorescence in situ hybridization (FISH).
10:21
Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers
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Cited by 18 •
2016
Multicolor fluorescence detection in droplet microfluidics typically involves bulky and complex epifluorescence microscope-based detection systems. Here we describe a compact and modular multicolor detection scheme that utilizes an array of optical fibers to temporally encode multicolor data collected by a single photodetector.
FAQs
Separating the two light signals allows the detector to measure fluorescence rather than the much shorter-wavelength light used for excitation. Filters or comparable optical separation components isolate the longer-wavelength emission, making the signal easier to associate with the labeled biological material. This distinction supports reliable measurement of fluorescent molecules, cells, or structures.
The selected excitation wavelength determines which fluorophore receives energy, while the emitted fluorescence appears at a longer wavelength after part of that energy is released. Choosing these wavelengths appropriately helps distinguish the signal from excitation light and can also separate fluorescent labels by color. The resulting spectral information supports detection of different biological targets.
Each signal characteristic provides a different type of biological information. Fluorescence intensity can indicate the presence or abundance of a labeled substance, location can show where molecules or structures occur, and color can help distinguish signals associated with different labels. Together, these readouts connect molecular labeling with cellular organization and distribution.
Fluorescent labels make selected molecules visible within biological samples, allowing their distribution and co-occurrence to be examined through detected signals. When fluorescence is measured by intensity, location, or color, researchers can relate labeled components to cellular structures or to one another. This makes the method useful for investigating biological organization and molecular interactions.
A typical workflow selects a fluorophore appropriate for the biological target, illuminates it with a suitable excitation wavelength, and collects the resulting longer-wavelength emission. Filters or detectors separate emission from excitation light, after which signal intensity, location, or color is assessed. The readout can then indicate the presence, abundance, or distribution of the labeled material.
Fluorescence detection is used across microscopy, protein analysis, nucleic acid analysis, cell-based assays, and diagnostic measurements. In microscopy, it can reveal labeled cellular structures; in molecular analyses, it can provide signals associated with proteins or nucleic acids. Cell-based and diagnostic applications use the same measurable fluorescence readouts to assess labeled biological material.