Fluorescence Video Microscopy
Fluorescence Video Microscopy - Related Videos
09:22
Introduction to Fluorescence Microscopy
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2023
Fluorescence microscopy is a very powerful analytical tool that combines the magnifying properties of light microscopy with visualization of fluorescence. Fluorescence is a phenomenon that involves absorbance and emission of a small range of light wavelengths by a fluorescent molecule known as a fluorophore. Fluorescence microscopy is accomplished in conjunction with the basic light microscope by the addition of a powerful light source, specialized filters, and a means of fluorescently...
08:52
Live-cell Video Microscopy of Fungal Pathogen Phagocytosis
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Cited by 22 •
2013
We describe methods for live-cell video microscopy of Candida albicans phagocytosis by macrophages. These methods enable stage-specific analysis of macrophage migration, recognition, engulfment and phagosome maturation and reveal novel aspects of phagocytosis.
14:10
Video-rate Scanning Confocal Microscopy and Microendoscopy
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Cited by 14 •
2011
The complete construction of a custom, real-time confocal scanning imaging system is described. This system, which can be readily used for video-rate microscopy and microendoscopy, allows for an array of imaging geometries and applications not accessible using standard commercial confocal systems, at a fraction of the cost.
10:16
Intravital Video Microscopy Measurements of Retinal Blood Flow in Mice
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Cited by 14 •
2013
Intravital microscopy can be used in animals to visualize and measure retinal vascular diameters, bloodstream velocities, and total retinal blood flow.
11:23
Lensless Fluorescent Microscopy on a Chip
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Cited by 8 •
2011
A lensless on-chip fluorescent microscopy platform is demonstrated that can image fluorescent objects over an ultra-wide field-of-view of e.g., >0.6-8 cm2 with <4μm resolution using a compressive sampling based decoding algorithm. Such a compact and wide-field fluorescent on-chip imaging modality could be valuable for high-throughput cytometry, rare-cell research and microarray-analysis.
FAQs
Contrast arises because fluorescent probes respond to illumination at an excitation wavelength and emit light at a longer wavelength. Optical filters separate this emitted signal from the excitation light before it reaches the camera. This separation allows researchers to visualize labeled cells, tissues, or molecules against surrounding material and follow where the fluorescent signal appears over time.
Sequential imaging preserves the order and timing of biological changes, rather than showing only one moment. Researchers can therefore observe movement, redistribution, division, signaling-related changes, or interactions as they unfold. The resulting time-resolved record supports interpretation of dynamic behavior and distinguishes processes that may appear identical in isolated static images.
Fluorescent probes provide the signal that identifies selected cells, structures, or molecules within a biological sample. Their labeled locations can then be followed across successive frames, linking molecular position with changing cellular behavior. This combination of molecular specificity and temporal information helps researchers examine protein localization, organelle movement, and other processes that cannot be resolved from unlabeled structural views alone.
A typical workflow begins by illuminating the fluorescently labeled biological material at the relevant excitation wavelength. The emitted light is collected through optical filters that select the longer-wavelength fluorescence, and a camera records successive images. Arranging these frames in sequence produces a time-lapse record that can be examined for movement, localization changes, division, or interactions.
Researchers choose this approach when the central question concerns biological change over time and the relevant cells, tissues, or molecules can be fluorescently labeled. It is suited to tracking protein localization, organelle movement, cell division, signaling, and interactions between living cells. The method is especially informative when a static image would conceal the order or progression of events.
Analysis can provide a time-resolved view of where fluorescent signals occur and how those locations change across successive images. Researchers can use the video to examine cellular behavior and perform quantitative analysis of observed dynamics. In biology, these outcomes help connect molecular or structural changes with processes such as movement, division, signaling, and cell-cell interaction.