Freezer Mill
Freezer Mill - Related Videos
14:00
Preparation of Cell Extracts by Cryogrinding in an Automated Freezer Mill
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Cited by 5 •
2021
We describe a reliable method for the preparation of whole cell extracts from yeast or other cells using a cryogenic freezer mill that minimizes degradation and denaturation of proteins. The cell extracts are suitable for purification of functional protein complexes, proteomic analyses, co-immunoprecipitation studies and detection of labile protein modifications.
10:09
Optimization of a Quantitative Micro-neutralization Assay
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Cited by 22 •
2016
This study describes an imaging-based micro-neutralization assay to analyze the antigenic relationships between viruses. The protocol employs a flatbed scanner and has four steps, including titration, titration quantitation, neutralization, and neutralization quantitation. The assay works well with current circulating influenza A(H1N1)pdm09, A(H3N2), and B viruses.
10:11
In-depth Physiological Analysis of Defined Cell Populations in Acute Tissue Slices of the Mouse Vomeronasal Organ
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Cited by 1 •
2016
Here, we describe a physiological approach that allows identification and in-depth analysis of a defined population of sensory neurons in acute coronal tissue slices of the mouse vomeronasal organ using whole-cell patch-clamp recordings.
08:07
Regulating Temperature in the Lab: Preserving Samples Using Cold
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2023
Preservation of laboratory samples, specimens, and reagents using extreme cold is routinely performed in biomedical research labs. This video will discuss some of the methods for keeping laboratory samples cold and will explain the correct cooling method to use for each experimental requirement. For example, cooling agents, such as ice and dry ice, are typically used when keeping samples cold during experiments. This video discusses the physical properties of the most commonly used cooling...
08:16
Ice Generation and the Heat and Mass Transfer Phenomena of Introducing Water to a Cold Bath of Brine
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Cited by 1 •
2017
Here, we present a protocol to demonstrate the generation of ice when water is introduced to a cold bath of brine, as a secondary refrigerant, at a range of temperatures well below the freezing point of water. It can be used as an alternative way of producing ice for industry.
FAQs
Within the sealed container, the sample and grinding media undergo repeated impact or agitation. This physical action breaks down material into a consistent, fine powder, including specimens whose hardness, fibrous structure, or biological complexity can make preparation difficult. Keeping the container closed during processing supports controlled sample handling before downstream extraction or biochemical analysis.
Cryogenic cooling limits heat generation during pulverization, which is important because biological constituents can be altered by warming. The low-temperature environment also limits oxidation and enzymatic degradation. As a result, temperature-sensitive components are better preserved for subsequent DNA, RNA, protein, or metabolite extraction, helping the prepared material remain suitable for molecular and biochemical measurements.
The method is particularly useful when a specimen is hard, fibrous, or biologically complex, because repeated mechanical treatment can reduce these materials to a fine powder. In biological work, that suitability extends across tissues, plant material, cells, and other specimens. This approach is therefore valuable when the material requires substantial mechanical disruption before analysis.
A consistent, fine powder provides downstream procedures with a more uniform starting material. This matters when samples are prepared for extracting DNA, RNA, proteins, or metabolites, and when they will enter molecular analyses or biochemical assays. Consistent preparation also supports reproducibility, allowing results to reflect the biology being studied rather than major differences in initial sample breakdown.
A preparation sequence begins by placing the specimen and grinding media in a sealed container. The container is then subjected to repeated impact or agitation while cryogenic cooling maintains low temperature. Processing continues until the material becomes a fine powder suitable for the planned extraction or assay. The resulting sample can then proceed to molecular or biochemical analysis.
In biology, freezer-milled material can serve as input for DNA, RNA, protein, or metabolite extraction. The prepared powder may support molecular analysis and biochemical assays, depending on the target component. Its value is greatest when the study requires disruption of tissues, plant material, cells, or other complex specimens while preserving temperature-sensitive components for downstream measurements.