Full-thickness Skin Excision
Full-thickness Skin Excision - Related Videos
07:59
Murine Full-thickness Skin Transplantation
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Cited by 41 •
2017
Murine full-thickness skin transplantation is a well-established model to study rejection in an alloimmune setting. Here, we provide a tutorial of each step involved in performing a BALB/c-->C57BL/6 full-thickness skin transplant.
08:35
Generation of a Three-dimensional Full Thickness Skin Equivalent and Automated Wounding
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Cited by 53 •
2015
The goal of this protocol is to build up a three-dimensional full thickness skin equivalent, which resembles natural skin. With a specifically constructed automated wounding device, precise and reproducible wounds can be generated under maintenance of sterility.
05:50
CUBIC-based Protein Expression Visualization: A Procedure for Visualizing Protein Expression in Full Thickness Mouse Skin Biopsies
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2023
This video describes the CUBIC-based visualization technique, a tissue clearing-dependent imaging technique for visual assessment of protein expression in whole-mount skin biopsies at single-cell resolution in 3D. This technically simple protocol allows the visualization of skin proteins without preparing skin sections.
01:54
Base Excision Repair
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2020
One of the common DNA damages is the chemical alteration of single bases by alkylation, oxidation, or deamination. The altered bases cause mispairing and strand breakage during replication. This type of damage causes minimal change to the DNA double helix structure and can be repaired by the base excision repair (BER) pathways. BER corrects damaged DNA sequences by removing the damaged base and restoring the original base sequence using the complementary strand as a template. The first step of...
08:35
Demonstration of the Rat Ischemic Skin Wound Model
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Cited by 32 •
2015
The rat, due to its size, availability, and rather docile behavior, has been utilized as a research model for many years. The goal of this protocol is to utilize the rat as an ischemic skin wound healing model to provide valuable insight into the pathophysiology of chronic wounds.
FAQs
Healing proceeds through coordinated hemostasis, inflammation, cell proliferation, re-epithelialization, extracellular matrix deposition, and tissue remodeling. These phases are biologically connected rather than isolated events: disruption of the barrier initiates the response, proliferative activity supports closure, and matrix changes help shape the later tissue outcome. Studying their sequence helps researchers distinguish delayed closure, altered immune activity, impaired regeneration, and excessive scar formation.
Removing the epidermis and entire dermis, with possible extension into subcutaneous tissue, produces a deeper and standardized injury than a limited surface disruption. That depth exposes biological differences in closure, angiogenesis, inflammation, matrix deposition, and remodeling. Consistent injury dimensions therefore improve comparisons among treatments, biomaterials, cell-based therapies, and disease conditions examined in experimental biology.
Variation in wound closure may reflect changes in re-epithelialization, cell proliferation, inflammation, extracellular matrix deposition, or tissue remodeling. Angiogenesis and immune responses can also influence the developing wound environment. Measuring wound area alongside histological changes allows investigators to connect the visible rate of closure with underlying tissue events rather than treating closure as a single undifferentiated outcome.
Two complementary outcome types are emphasized: wound-area measurements and histological assessment. Wound area provides a quantitative indication of closure over the course of healing, while histology reveals tissue-level changes associated with inflammation, proliferation, re-epithelialization, matrix deposition, and remodeling. Together, these measurements support comparisons between interventions and help determine whether apparent closure corresponds to broader biological improvement.
The model provides a standardized open wound for evaluating biomaterials, cell-based therapies, and topical treatments. Investigators can compare treated and untreated healing outcomes using wound-area measurements and histological changes. This design helps determine whether an intervention influences closure, angiogenesis, immune responses, scar formation, or tissue regeneration, making the procedure useful for linking treatment effects to specific repair processes.
Its value extends to studying how tissue repair is coordinated across barrier disruption, immune activity, vascular development, cell proliferation, matrix formation, and remodeling. The model also supports investigation of scar formation and tissue regeneration, including differences associated with disease conditions. Consequently, researchers can examine both the visible outcome of healing and the biological mechanisms that produce that outcome.