Fura Ff Jc-1
Fura Ff Jc-1 - Related Videos
12:15
Calcium Imaging of Cortical Neurons using Fura-2 AM
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Cited by 108 •
2009
Calcium signals play a key role in many cellular processes including gene expression, survival and differentiation. Here we demonstrate how to perform calcium imaging using Fura-2 AM. Calcium imaging is a valuable tool to study the regulation of intracellular calcium in real time and its regulation of signaling cascades.
04:17
Formalin-Fixed Paraffin Embedded (FFPE) Tissue Preservation: A Method for Studying Tissue Morphology
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2023
The following video describes how to preserve morphology of a tissue with formalin fixation and paraffin embedding.
04:33
Extracellular DNA Staining: A Method to Identify Extracellular DNA in FFPE Tissue Sections
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2023
This video describes the technique of staining extracellular DNA in FFPE tissue sections. The stained extracellular DNA can be quantitated to determine the efficacy of any therapeutic treatment that leads to the death of cancer cells.
02:43
Deparaffinization and Rehydration of Tissue Sections: A Two-step Procedure to Remove Paraffin Wax from FFPE Tissue Slides Followed by Tissue Rehydration
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2023
In this video, we demonstrate the processing of FFPE or formalin-fixed paraffin-embedded sections obtained from gastrointestinal tumor tissue. The deparaffinization and rehydration steps allow the processing of the tissue specimen for subsequent analytical applications.
03:53
FFPE Tissue Pretreatment for RNA CISH: A Procedure to Process Formalin-Fixed Paraffin-Embedded Tissue Sections for RNA Chromogenic In Situ Hybridization
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2023
This video demonstrates the steps involved in the pretreatment of FFPE tissue specimens for RNA chromogenic in situ hybridization. The pretreatment process helps maintain nucleic acid integrity within the cells and overall tissue morphology.
FAQs
Fura-FF reports calcium through a binding-dependent fluorescence change, so the measured signal reflects interaction between the probe and intracellular calcium rather than mitochondrial status. Changes in fluorescence can therefore be followed over time to examine calcium signaling within living cells. This makes the indicator useful for linking altered calcium behavior to broader changes in cell physiology.
JC-1 distinguishes mitochondrial states through its distribution between green-emitting monomers and red-emitting aggregates. As mitochondrial membrane potential increases, the probe accumulates in energized mitochondria and forms more aggregates, shifting the fluorescence pattern toward red emission. This response provides an optical readout of mitochondrial activity and helps identify changes associated with cellular stress.
The probes measure different aspects of cell function. Fura-FF follows intracellular calcium behavior through fluorescence changes caused by calcium binding, whereas JC-1 reflects mitochondrial membrane potential through a green-to-red emission shift. Using the appropriate indicator helps distinguish altered calcium signaling from changes in mitochondrial activity when investigating cellular stress or injury.
A measurement workflow selects the probe according to the cellular variable of interest, then records fluorescence with either fluorescence microscopy or a plate-based assay. Imaging supports observation in living cells, while plate formats support quantitative analysis across samples. The resulting fluorescence changes can be compared with experimental treatments to evaluate effects on calcium or mitochondrial function.
These indicators are useful when researchers need to connect physiological changes with disease mechanisms or experimental treatments. Fura-FF can track calcium signaling alterations, while JC-1 can indicate changes in mitochondrial activity associated with cellular stress and apoptosis. Together, such measurements help characterize how drug-induced cellular injury affects distinct but related aspects of cell function.
Fluorescence data can provide quantitative evidence of changing intracellular calcium levels or mitochondrial membrane potential. In live-cell studies, those readouts help reveal altered calcium signaling, impaired mitochondrial activity, cellular stress, and apoptosis-related changes. Interpreting the signal in relation to a treatment or disease model allows researchers to connect molecular events with observable changes in cellular physiology.