Gal4-vp16
Gal4-vp16 - Related Videos
13:34
Gene Trapping Using Gal4 in Zebrafish
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Cited by 19 •
2013
This protocol describes the method of gene trap insertional mutagenesis using Gal4-VP16 as the primary reporter and GFP/RFP as secondary reporters in zebrafish. Approximately one in ten high-expressing F0 fish yield gene trap progeny co-expressing GFP and RFP. The screening procedure can be readily scaled to adapt to the size of the laboratory performing the insertional mutagenesis screen.
06:29
Using the GAL4-UAS System for Functional Genetics in Anopheles gambiae
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Cited by 11 •
2021
The bipartite GAL4-UAS system is a versatile tool for modification of gene expression in a controlled spatiotemporal manner which permits functional genetic analysis in Anopheles gambiae. The procedures described for using this system are a semi-standardized cloning strategy, sexing and screening of pupae for fluorescent protein markers and embryo fixation.
08:18
Targeting Olfactory Bulb Neurons Using Combined In Vivo Electroporation and Gal4-Based Enhancer Trap Zebrafish Lines
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Cited by 8 •
2011
The temporal and spatial resolution of genetic manipulations determines the spectrum of biological phenomena that they can perturb. Here we use temporally and spatially discrete in vivo electroporation, combined with transgenic lines of zebrafish, to induce expression of a GFP transgene specifically in neurons of the developing olfactory bulb.
08:52
Live Imaging of Innate Immune and Preneoplastic Cell Interactions Using an Inducible Gal4/UAS Expression System in Larval Zebrafish Skin
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Cited by 9 •
2015
Studying the earliest events of preneoplastic cell progression and innate immune cell interaction is pivotal to understand and treat cancer. Here we describe a method to conditionally induce epithelial cell transformations and the subsequent live imaging of innate immune cell interaction with HRASG12V expressing skin cells in zebrafish larvae.
03:27
Inducible Tet-On Regulatable System-Based Gene Expression In Vivo: A Tetracycline-Induced Gene Expression System for Target Gene Transcription Activation in Mouse Model
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2025
This video demonstrates the tetracycline, or Tet-On, gene expression system in a mouse model. In the presence of doxycycline, a tetracycline derivative, the Tet-On system consisting of reverse tetracycline-controlled transactivator protein becomes activated and expresses the gene of interest.
FAQs
Specificity comes from the Gal4 portion recognizing UAS sequences, while transcriptional strength depends on the attached VP16 activation domain. After binding, VP16 recruits transcriptional coactivators and RNA polymerase-associated machinery. This division of labor lets investigators direct activation toward a chosen UAS-linked gene rather than relying only on the gene’s native regulatory context.
UAS placement determines which gene responds to the activator. When a reporter or other gene is linked to UAS sequences, Gal4-vp16 binding can promote transcription of that linked target through VP16-mediated recruitment. This modular arrangement allows the same activator to be paired with different UAS-linked genes, supporting controlled comparisons of gene activity or regulatory-element behavior.
Control arises from the requirement for two compatible components: Gal4-vp16 must be present, and the target gene must be linked to recognized UAS sequences. This pairing restricts the engineered activation system to genes placed under UAS control. Researchers can therefore examine selected transcriptional responses without treating every gene in the biological system as an equivalent target.
A basic experimental design combines the Gal4-vp16 activator with a gene placed downstream of UAS sequences. That linked gene may encode a reporter for visualizing activity or another functional product for manipulating cell behavior. Researchers then examine transcription from the configured target, using the modular pairing to connect the activator with a specific experimental readout.
UAS-linked reporters provide an observable output of transcriptional activation. When Gal4-vp16 engages the UAS control sequences, expression of the linked reporter can reveal where or when the system is active in the biological preparation. This approach helps investigators visualize gene activity and evaluate how regulatory elements behave under defined experimental conditions.
In model organisms, the system supports studies of development, cell lineage, neural circuits, and tissue-specific gene function. Investigators can use UAS-linked reporters to visualize activity or connect the system to genes that alter cell behavior. These applications make it useful for relating controlled transcriptional activation to patterns of development and specialized tissue functions.