Gene Amplification
Gene Amplification - Related Videos
02:48
Loop-Mediated Isothermal Amplification for Bacterial Gene Detection
0 Views •
2025
Source: Domesle, K. J., et al. Loop-Mediated Isothermal Amplification for Screening Salmonella in Animal Food and Confirming Salmonella from Culture Isolation. J. Vis. Exp. (2020).This video demonstrates the loop-mediated isothermal amplification (LAMP) technique for detecting a target bacterial gene by amplifying DNA at a constant temperature. The reaction mixture is prepared by adding an isothermal master mix, specific primers for the target gene, and samples containing DNA templates. The...
09:44
Rapid Amplification of cDNA Ends
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2023
Source: Pablo Sanchez Bosch2, Sean Corcoran2 and Katja Brückner1,2,3 1Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research 2Department of Cell and Tissue Biology, 3Cardiovascular Research Institute, University of California San Francisco, San Francisco, CA, USA Rapid Amplification of cDNA Ends (RACE) is a technique that allows amplification of full-length cDNA from mRNA by extending to the 3’ or 5’ end, even without prior knowledge of the sequence (Frohman et al.,...
02:41
Amplification of a Suicide Plasmid in a Bacterial Host
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2025
Source: Tomás, J. M., et al. Generation of Null Mutants to Elucidate the Role of Bacterial Glycosyltransferases in Bacterial Motility. J. Vis. Exp. (2022).This video demonstrates the initiator-dependent amplification of a suicide plasmid in E. coli. The plasmid, which carries an antibiotic resistance gene, replicates only in the presence of an initiator protein encoded by the host. After electroporation and incubation in recovery medium, the mixture is plated on antibiotic-containing agar.
10:12
Protein Misfolding Cyclic Amplification of Prions
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Cited by 7 •
2012
Protein misfolding cyclic amplification (PMCA) is an in vitro assay for the study of prion conversion and strain and species barriers. It can also be used as a prion detection assay.
11:34
Building Up a High-throughput Screening Platform to Assess the Heterogeneity of HER2 Gene Amplification in Breast Cancers
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Cited by 19 •
2017
Heterogeneous distribution of HER2-positive cells can be observed in a subset of breast cancers and generates clinical dilemmas. Here, we introduce a reliable and cost-effective protocol to define, quantify, and compare HER2 intra-tumor genetic heterogeneity in a large series of heterogeneously processed breast cancers.
FAQs
The cellular and laboratory forms differ in control and purpose. In cells, repeated replication or genomic rearrangements can duplicate larger regions, potentially changing gene dosage within an organism. PCR, by contrast, targets a selected sequence through programmed cycles, producing material for analysis rather than changing the genome of the source cells.
Increased gene dosage can change how much protein a gene produces, making amplification biologically consequential rather than merely structural. Altered protein production has been linked with tumor development. This relationship makes duplicated genomic regions relevant to biology and motivates genetic investigation into how extra gene copies may be associated with cellular and disease-related processes.
Each PCR cycle separates the DNA strands during denaturation, allows primers to anneal to the target sequence, and uses a heat-stable polymerase for DNA synthesis. Repeating this sequence increases the amount of the selected DNA region. The cycle-based design makes it possible to obtain enough material for sensitive genetic analysis.
Primers provide the sequence-specific annealing step that directs attention to the region being analyzed, while the heat-stable polymerase carries out DNA synthesis after the strands separate. Their coordinated use allows PCR to repeatedly copy a selected target through changing temperature conditions without losing polymerase activity during the process.
Researchers apply gene amplification when a target sequence must be detected, examined, or produced in greater quantity. Supported uses include pathogen detection, mutation identification, nucleic-acid quantification, cloning, sequencing, and diagnostic testing. These applications make amplification useful when the original genetic material is difficult to analyze directly or is present in limited amounts.
Amplified material can provide evidence that a pathogen or particular genetic sequence is present, support identification of mutations, and enable quantification of nucleic acids. It can also supply abundant DNA for cloning and sequencing. In diagnostic contexts, these outputs help connect a molecular signal with genetic testing or disease-related investigation.