Gene Perturbation
Gene Perturbation - Related Videos
10:37
Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform
0 Views •
Cited by 6 •
2016
We describe the use of a multiplexed high-throughput gene expression platform that quantitates gene expression by barcoding and counting molecules in biological substrates without the need for amplification. We used the platform to quantitate microRNA (miRNA) expression in whole blood in subjects with and without irritable bowel syndrome.
07:07
Optogenetic Perturbation of Neural Activity with Laser Illumination in Semi-intact Drosophila Larvae in Motion
0 Views •
Cited by 13 •
2013
Here we describe a protocol for optogenetic manipulation of motoneuronal activity while monitoring changes in motor output (muscle contraction) in semi-intact Drosophila larvae using lasers within a conventional confocal microscope. This technique enables researchers to achieve local perturbation of neural activity in a few neuromeres to elucidate the dynamics of motor circuits.
07:49
Application of Consistent Massage-Like Perturbations on Mouse Calves and Monitoring the Resulting Intramuscular Pressure Changes
0 Views •
Cited by 5 •
2019
Here we describe the protocols for applying defined mechanical loads to mouse calves and for monitoring the concomitant intramuscular pressure changes. The experimental systems that we have developed can be useful for investigating the mechanism behind the beneficial effects of physical exercise and massage.
09:20
Perturbing Endothelial Biomechanics via Connexin 43 Structural Disruption
0 Views •
Cited by 6 •
2019
Here, we present a mechanics-based protocol to disrupt the gap junction connexin 43 and measure the subsequent impact this has on endothelial biomechanics via observation of tractions and intercellular stresses.
00:07
Accurate Determination of the Equilibrium Surface Tension Values with Area Perturbation Tests
0 Views •
Cited by 1 •
2019
Two protocols for determining the equilibrium surface tension (EST) values using the emerging bubble method (EBM) and the spinning bubble method (SBM) are presented for a surfactant-containing aqueous phase against air.
FAQs
These approaches change gene function through distinct intervention types. CRISPR-based editing can modify a gene sequence, RNA interference can reduce gene activity, and targeted activation can increase it. Comparing these outcomes helps researchers distinguish effects caused by loss, reduction, elevation, or sequence-level modification of function, providing a more detailed view of how a gene contributes to cellular traits.
A genetic change becomes biologically informative only when it can be linked to an observable cellular or organismal outcome. Measuring phenotypic changes allows researchers to connect altered gene function with specific traits or processes. This evidence supports conclusions about gene roles and helps identify relationships between genetic activity and broader biological responses.
Perturbing one gene and examining resulting cellular changes can reveal how its activity relates to other biological processes. When these relationships are considered across multiple genes, researchers can characterize regulatory networks and pathways rather than isolated effects. This systems-level perspective helps explain coordinated control of cellular traits, development, disease-related processes, and cellular responses.
Researchers can compare disruption, reduced activity, increased activity, and sequence modification to determine whether a biological outcome depends on the amount or form of gene function. These contrasting perturbations can separate effects associated with insufficient activity from those associated with enhanced or altered activity, strengthening interpretation of how a gene influences a cellular process.
A study generally selects a gene or pathway of interest, applies an approach such as CRISPR-based editing, RNA interference, or targeted activation, and then measures the resulting phenotypic changes. Researchers interpret those outcomes in relation to the biological process under investigation. This workflow connects a deliberate genetic intervention with evidence about gene function.
Gene perturbation is useful when researchers need evidence that a particular gene contributes to a trait, pathway, or disease-related process. In functional genomics, it helps map gene function across biological systems, while target validation uses observed perturbation outcomes to assess whether a gene is relevant for further investigation or potential therapeutic strategy development.